合成了枝状聚乙二醇醚链取代的新型不对称五甲川吲哚菁荧光染料,利用1H NMR、HRMS等技术手段表征了化合物的结构,并测定了染料的荧光性能、光稳定性,标示了牛血清白蛋白.结果表明,该染料的最大吸收波长为657 nm,最大荧光发射波长为671 nm,荧光量子产率为0.24,经过8h的光照反应,染料有4.4％产生光降解,溶于水,n(染料):n(牛血清白蛋白)=2:1时,标记蛋白质的染料/蛋白质(D/P)值达1.57.
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