Sirtuin蛋白是一类称为依赖烟酰胺腺嘌呤二核苷酸(NAD)的组蛋白去乙酰化酶,共有7个成员,均是潜在的疾病治疗靶点.然而,目前的荧光筛选方法,只适用于SIRT1～SIRT3.因此,根据SIRT5的新酶活,设计、合成了针对SIRT5的荧光标记多肽(ISGASE (SuK)-AMC),并通过LC-MS和荧光检测证明了该荧光标记多肽能应用于SIRT5的活性筛选.
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