建立了高效液相色谱法同时测定乳及乳制品中安赛蜜、苯甲酸、糖精钠、山梨酸和阿斯巴甜的方法.通过加入适量沉淀剂除去样品中绝大部分蛋白质后,采用C18色谱柱分离,以甲醇-0.05 mol/L 磷酸二氢钾溶液为流动相梯度洗脱,用二极管阵列检测器于230 nm波长处检测安赛蜜、苯甲酸和山梨酸,于210 nm波长处检测糖精钠和阿斯巴甜.被测物的回收率为96.0% ～103.5% ,精密度(以相对标准偏差(RSD)计)为1.93% ～2.76% ,安赛蜜、苯甲酸、糖精钠、山梨酸和阿斯巴甜的检出限分别为1.0,1.0,0.5,1.0,1.5 μg/g.该方法可用于乳及乳制品中这5种添加剂的同时测定.
A method for simultaneous determination of acesulfame,benzoic acid,sodium saccharin,sorbic acid,and aspartame in milk and dairy products using high performance liquid chromatography (HPLC) was developed.The proteins in milk and dairy products were mostly eliminated by the precipitators.Three aseptics and two sweeteners were separated on a C18 column with the mobile phase of methanol-0.05 mol/L potassium dihydrogen phosphate under gradient elution.With a diode array detector,acesulfame,benzoic acid,and sorbic acid were detected at 230 nm and sodium saccharin and aspartame were detected at 210 nm.The recoveries were 96.0% -103.5% with the relative standard deviations (RSDs) in the range of 1.93% -2.76% .The detection limits of acesulfame,benzoic acid,sodium saccharin,sorbic acid and aspartame were 1.0,1.0,0.5,1.0,and 1.5 μg/g,respectively.This method can be used for the routine analysis of these additives in milk and dairy products.
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