以高效液相色谱手性固定相法对非洛地平(FEL)进行手性拆分.分别采用两种类型的纤维素-三(对甲基苯甲酸酯)手性柱Chiralcel OJ-R和Chiralcel OJ-H进行比较实验,以正己烷-异丙醇(90:10,v/v)为流动相,考察了流动相、柱温对保留及手性拆分的影响.实验显示,两柱拆分FEL的van't Hoff图均发生了转折,在高温区域为焓驱动,在低温区域为熵驱动.两柱在温度升高时拆分FEL的分离度均提高,其中OJ-H的分离度优于OJ-R.两种手性柱对FEL具有相似的拆分机理.
参考文献
| [1] | Cancelliere G,D'Acquarica I,Gasparrini F,et al.Pham Sci Tech Today,1999,2(12):484 |
| [2] | Chen L R.Chiral Separation by Liquid Chromatography.Beijing:Science Press (陈立仁.液相色谱手性分离.北京:科学出版社),2006 |
| [3] | Han X Q,Wei Y,Liu Y H,et al.Chinese Journal of Chromatography (韩小茜,魏燕,刘艳华,等.色谱),2008,26(5):631 |
| [4] | Chen Y Q,Ma Z,An F,et al.Chinese Journal of Chromatography (陈玉青,马郑,安芳,等.色谱),2008,26(5):643 |
| [5] | Huang H,Jin J Y,Lee W.Chinese Journal of Chromatography (黄虎,金京玉,李元宰.色谱),2009,27(4):467 |
| [6] | Gu Y,Wang G J,Sun J G.West China Journal of Pharmaceutical Sciences (顾轶,王广基,孙建国.华西药学杂志),2006,21(4):372 |
| [7] | Karlsson A,Pettersson K,Hernqvist K.Chirality,1995,7(3):147 |
| [8] | Boatto G,Nieddu M,Faedda M V,et al.Chirality,2003,15(6):494 |
| [9] | Chen Z Y,Yao T W,Zeng S.Chinese Pharmaceutical Journal (陈仲益,姚彤炜,曾苏.中国药学杂志),2007,42(7):544 |
| [10] | Soons P A,Roosemalen M C M,Breimer D D.J Chromatogr B,1990,528:343 |
| [11] | Lindmark B,Ahnoff M,Persson B A.J Pharm Biomed Anal,2002,27:489 |
| [12] | O'Brien T,Crocker L,Thompson R,et al.Anal Chem,1997,69(11):1999 |
| [13] | Weng W,Yao B X,Chen X Q,et al.Progress in Chemistry (翁文,姚碧霞,陈秀琴,等.化学进展),2006,18(7/8):1056 |
| [14] | Kusters,E,Loux V,Schmid E.J Chromatogr,1994,666(1/2):421 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%