建立了一种离子色谱定量检测酒曲发酵液中河豚毒素的分析方法.样品经乙腈(含0.1%磷酸)溶液提取和阳离子交换柱净化后,采用离子交换色谱柱分离和紫外检测.在优化的条件下,酒曲样品中的河豚毒素在10～100mg/L内呈良好的线性关系(r2=0.997),加标回收率为90%～103%,相对标准偏差小于4.9%,检出限(信噪比为3)为1.0 mg/L.结果表明,该方法能达到定量检测的目的.将该方法应用于实际样品的检测,验证了方法的可靠性.河豚毒素初步降解实验发现,随着时间的推移,酒曲中河豚毒素的含量逐渐减少,表明酒曲发酵液对河豚毒素的降解效果显著.
参考文献
| [1] | Su J,Zhang N,Jiang L L.Fishery Modernization(苏捷,张农,姜琳琳.渔业现代化),2007,34(3):34 |
| [2] | Wang G N,Xie L P,Zhang R Q.Journal of Marine Science Bulletin(王冠男,谢丽萍,张荣庆.海洋通报),2007,26(1):107 |
| [3] | Duan F M,Xie X L,Zhu B P.Chinese Journal of Health Laboratory Technology(段发淼,谢心磊,朱宝平.中国卫生检验杂志),2000,10(4):463 |
| [4] | Zhou Y,Li Y S,Pan FG,et al.Journal of Medical Colleges of PLA,2007,22(6):347 |
| [5] | Zhang X Y,Cai X X.Chinese Journal of Analytical Chemistry(张秀尧,蔡欣欣.分析化学),2009,12(37):1829 |
| [6] | Shoji Y,Yotsu-Yamashita M,Miyatawa T,et al.Anal Biochem,2001,290(1):10 |
| [7] | Hong N H,Jie L,H L L.Toxicon,2008,51(5):774 |
| [8] | Ito K,Okabe S,Asakawa M,et al.Toxicon,2006,48(4):620 |
| [9] | Asakawa M,Toyoshima T,Ito K,et al.Toxicon,2003,41(7):747 |
| [10] | Tsai YH,Hwang D F,Cheng CA,et al.J Chromatogr B,2006,832(1):75 |
| [11] | Li S P,Jiao X A,Huang J L,et al.Journal of Yangzhou University:Agricultural and Life Science Edition(李世平,焦新安,黄金林,等.扬州大学学报:农业与生命科学版),2004,25(2):58 |
| [12] | Lin W L,Huang H L,Chen S X.Journal of Huaqiao University:Natural Science(林文銮,黄惠莉,陈少欣.华侨大学学报:自然科学版),1999,20(4):412 |
| [13] | Yao J H,Tan Z J,Zhou D Q,et al.Chinese Journal of Chromatography(姚建华,谭志军,周德庆,等.色谱),2010,28(4):363 |
| [14] | Cui J Z,Shen X Y,Gong Q L,et al.Chinese Journal of Chromatography(崔建洲,申雪艳,宫庆礼,等.色谱),2006,24(3):317 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%