建立了气相色谱-串联质谱法测定鹿茸保健品中11种性激素的分析方法.鹿茸中的性激素经固相萃取富集和净化,经七氟丁酸酐衍生处理.采用DB-5色谱柱(30 m×0.25 mm,0.25μm)、非线性梯度升温程序分离,在串联质谱多反应监测(MRM)模式下检测,外标法定量,实现了11种性激素的有效分离.11种性激素的检出限为1.0～5.0μg/kg,线性相关系数为0.991 6～0.999 9,平均回收率为67.4％～99.1％,相对标准偏差为2.6％～13％.该方法准确,可靠,可满足鹿茸保健品中性激素含量的测定和确证.
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