毛细管电泳以其分析速度快、分离效率高、操作简便、能够实现高通量而获得了广泛的应用,但由于检测窗口小而导致其检测灵敏度低.为了提高检测灵敏度,目前已发展了多种毛细管电泳在线聚焦和样品预浓缩技术,如场放大样品堆积、pH调节浓缩、胶束电动毛细管色谱、等速电泳等.这些技术由于能够在毛细管内同时实现样品的聚焦和分离、操作简便而获得J广泛的兴趣和关注.本文针对毛细管电泳的在线聚焦的原理、技术和应用做一简要的介绍和总结.
参考文献
| [1] | Timerbaev A.J Sep Sci,2008,31(11):2012 |
| [2] | Timerbaev A.Electrophoresis,2007,28 (19):3420 |
| [3] | Timerbaev A.Electrophoresis,2010,31 (1):192 |
| [4] | Foret F.Electrophoresis,2009,30(S1):S34 |
| [5] | Kasicka V.Electrophoresis,2006,27 (1):142 |
| [6] | Lu H.Anal Methods,2011,3(3):488 |
| [7] | Prokhorova A,Shapovalova E,Shpigun O.J Pharm Biomed Anal,2010,53(5):1170 |
| [8] | Fonslow B R,Yates J R.J Sep Sci,2009,32(8):1175 |
| [9] | Kleparnik K,Bocek P.Chem Rev,2007,107(11):5279 |
| [10] | Subirats X,Blaas D,Kenndler E.Electrophoresis,2011,32(13):1579 |
| [11] | Huang W,Ai F,Wang Z,et al.J Chromatogr B,2008,866(1/2):104 |
| [12] | Garcia-Canas V,Cifuentes A.Electrophoresis,2008,29(1):294 |
| [13] | Suntornsuk L.Anal Bioanal Chem,2010,398(1):29 |
| [14] | Ha P,Hoogmartens J,Van Schepdael A.J Pharm Biomed Anal,2006,41(1):1 |
| [15] | Scriba G K E.J Pharm Biomed Anal,2011,55(4):688 |
| [16] | Tagliaro F,Pascali J,Fanigliulo A,et al.Electrophoresis,2010,31(1):251 |
| [17] | Ali I,Gupta V,Aboul-Enein H.Crit Rev Anal Chem,2008,38(3):132 |
| [18] | Mahugo S C,Sosa F Z,Torres P M,et al.Molecules,2009,14(1):298 |
| [19] | Tankiewicz M,Fenik J,Biziuk M.Trac-Trend Anal Chem,2010,29(9):1050 |
| [20] | Kleparnik K,Bocek P.BioEssays,2010,32(3):218 |
| [21] | Breadmore M,Thabano J,Dawod M,et al.Electrophoresis,2009,30(1):230 |
| [22] | Sueyoshi K,Kitagawa F,Otsuka K.J Sep Sci,2008,31(14):2650 |
| [23] | Mala Z,Gebauer P,Bocek P.Electrophoresis,2011,32(1):116 |
| [24] | Simpson S,Quirino J,Terabe S.J Chromatogr A,2008,1184(1/2):504 |
| [25] | Aebersold R,Morrison H.J Chromatogr A,1990,516 (1):79 |
| [26] | Britz-McKibbin P,Chen D.Anal Chem,2000,72(6):1242 |
| [27] | Britz-McKibbin P,Bebault G,Chen D.Anal Chem,2000,72(8):1729 |
| [28] | Silva M.Electrophoresis,2011,32(1):149 |
| [29] | Silva M.Electrophoresis,2009,30(1):50 |
| [30] | Aranas A,Guidote A,Quirino J.Anal Bioanal Chem,2009,394(1):175 |
| [31] | Quirino J P,Kim J B,Terabe S.J Chromatogr A,2002,965(1/2):357 |
| [32] | Gebauer P,Mala Z,Bocek P.Electrophoresis,2011,32(1):83 |
| [33] | Hou X L,Deng D L,Wu X,et al.J Chromatogr A,2010,1217(35):5622 |
| [34] | He H,Lv X,Yu Q,et al.Talanta,2010,82(4):1562 |
| [35] | Li H,Wang P H,Li C,et al.Microchem J,2008,89(1):34 |
| [36] | Yu L S,Xu X Q,Huang L,et al.Electrophoresis,2009,30(4):661 |
| [37] | Zinellu A,Sotgia S,Murtas V D,et al.Anal Bioanal Chem,2011,399(3):1181 |
| [38] | Zinellu A,Sotgia S,Campesi I,et al.Talanta,2011,84(3):931 |
| [39] | Lian D S,Zhao S J.Biotechnology (连冬生,赵树进.生物技术),2009,19(6):57 |
| [40] | Yeh H,Yang Y,Ko J,et al.Electrophoresis,2008,29(17):3649 |
| [41] | Li J,Jiang Y.Biomed Chromatogr,2010,24(2):186 |
| [42] | Zheng X,Lu M,Zhang L,et al.Talanta,2008,76(1):15 |
| [43] | Weng Q F,Xu G W,Yuan K L,et al.J Chromatogr B,2006,835(1/2):55 |
| [44] | Xu L,Basheer C,Lee H K.J Chromatogr A,2010,1217(39):6036 |
| [45] | Honegr J,Safra J,Polasek M,et al.Chromatographia,2010,72(9/10):885 |
| [46] | Bail(o)n-Pérez M I,García-Campa(n)a A M,Cruces-Blanco C,et al.Electrophoresis,2007,28 (22):4082 |
| [47] | Li P,Duan J,Hu B.Electrophoresis,2008,29(14):3081 |
| [48] | Herrera-Herrera A V,Ravelo-Perez L M,Hernandez-Borges J,et al.J Chromatogr A,2011,1218(31):5352 |
| [49] | Li Y,He Y Z.Chinese Journal of Chromatography(李云,何友昭.色谱),2005,23(1):100 |
| [50] | Kitagawa S,Noji A,Matushita N,et al.Electrophoresis,2011,32(12):1480 |
| [51] | Zhu Z F,Yan N,Zhou X,et al.J Sep Sci,2009,32(20):3481 |
| [52] | Zhang Z X,Zhang X W,Wang J J,et al.Anal Bioanal Chem,2008,390(6):1645 |
| [53] | Zhang Z C,Wang J H,Hui L M,et al.J Chromatogr A,2011,1218(31):5336 |
| [54] | Ramsay L M,Cermak N,Dada O O,et al.Anal Bioanal Chem,2011,400(7):2025 |
| [55] | Mohan D,Lee C S.Electrophoresis,2002,23(18),3160 |
| [56] | Wang T T,Ma J F,Wu S B,et al.J Chromatogr B,2011,879(11/12):804 |
| [57] | Wei J A,Gu X,Wang Y,et al.Electrophoresis,2011,32(2):230 |
| [58] | Liu H C,Yang C,Yang Q,et al.J Chromatogr B,2005,817(1):119 |
| [59] | Han B,Wang P L,Zhang L H,et al.Chinese Journal of Chromatography(韩彬,王平利,张丽华,等.色谱),2009,27(4):383 |
| [60] | Lin J,Tan Q,Wang S.J Sep Sci,2011,34(14):1696 |
| [61] | Weiss N,Hayes M,Garcia A,et al.Langmuir,2011,27(1):494 |
| [62] | Egatz-Gomez A,Thormann W.Electrophoresis,2011,32(12):1433 |
| [63] | Zhao Y,Lunte C.Anal Chem,1999,71(18):3985 |
| [64] | Amett S,Lunte C.Electrophoresis,2003,24(11):1745 |
| [65] | Hoque M E,Amett S D,Lunte C E.J Chromatogr B,2005,827(1):51 |
| [66] | Gillogly J A,Lunte C E.Electrophoresis,2005,26 (3):633 |
| [67] | Wang S,Yang P,Zhao X.Chromatographia,2009,70(9/10):1479 |
| [68] | Wu Y,Liu J,Deng Z,et al.J Sep Sci,2010,33(19):3068 |
| [69] | Baidoo E E K,Benket P I,Neususs C,et al.Anal Chem,2008,80(9):3112 |
| [70] | Ye H,Xia S,Lin W,et al.Electrophoresis,2010,31(20):3400 |
| [71] | Kazarian A A,Hilder E F,Breadmore M C.Analyst,2010,135(8):1970 |
| [72] | Jaafar J,Irwan Z,Ahamad R,et al.J Sep Sci,2007,30(3):391 |
| [73] | Zhang X,Xu S,Sun Y,et al.Chromatographia,2011,73(11/12):1217 |
| [74] | Arnett S,Lunte C.Electrophoresis,2007,28(20):3786 |
| [75] | Hasan M,Park S,Oh E,et al.J Sep Sci,2010,33(23/24):3701 |
| [76] | Yu L J,Li S F Y.Electrophoresis,2005,26 (22):4360 |
| [77] | Cao C X,FanLY,Zhang W.Analyst,2008,133(9):1139 |
| [78] | Cao C X,Zhang W,Qin W H,et al.Anal Chem,2005,77(4):955 |
| [79] | Cao C X,He Y Z,Li M,et al.Anal Chem,2002,74(16):4167 |
| [80] | Wang X,Zhang W,Fan L Y,et al.Anal Chim Acta,2007,594 (2):290 |
| [81] | Li M,Fan L Y,Zhang W,et al.Anal Bioanal Chem,2007,387(8):2719 |
| [82] | Wang Q L,Fan L Y,Zhang W,et al.Anal Chim Acta,2006,580(2):200 |
| [83] | Terabe S,Otsuka K,Ichikawa K,et al.Anal Chem,1984,56(1):111 |
| [84] | Shen H J,Lin C H.Electrophoresis,2006,27(5/6):1255 |
| [85] | Cao J,Dun W.Talanta,2011,84(1):155 |
| [86] | Chen Y,Zhang L,Cai Z,et al.Analyst,2011,136(9):1852 |
| [87] | Wang C,Han D,Wang Z,et al.Anal Chim Acta,2006,572(2):190 |
| [88] | Liu F K.J Chromatogr A,2008,1215(1/2):194 |
| [89] | Wang C,Wang Z,Han D D,et al.Anal Lett,2006,39(9):1927 |
| [90] | Lin S C,Lin S W,Chen J M,et al.Talanta,2010,82(2):653 |
| [91] | Huang C W,Jen H P,Wang R D,et al.J Chromatogr A,2006,1110(1/2):240 |
| [92] | Li L J,Wu F M,Yu L B,et al.Chinese Journal of Analysis Laboratory(李利军,吴峰敏,喻来波,等.分析试验室),2008,27(12):55 |
| [93] | Yan Y N,Wang L J,Yang G L,et al.Chinese Journal of Chromatography(闫永娜,王利娟,杨更亮,等.色谱),2009,27 (6):860 |
| [94] | Aranas A T,Guidote A M Jr,Haddad P R,et al.Talanta,2011,85(1):86 |
| [95] | Zhang S,Yin X,Yang Q,et al.Anal Bioanal Chem,2011,401(3):1071 |
| [96] | Zhang S,LiC,Song S,et al.Anal Methods,2010,2(1):54 |
| [97] | Sun J,Chen G H,Wang K,et al.Chinese Journal of Analytical Chemistry(孙娟,陈冠华,王坤,等.分析化学),2010,38(8):1151 |
| [98] | Wang Q,Yang G L,Zhang L.Chinese Journal of Chromatography(王全,杨更亮,张骊.色谱),2007,25(3):341 |
| [99] | Tsai C H,Chan P H,Lin C H,et al.Electrophoresis,2006,27(23):4688 |
| [100] | Tsai C H,Lin J D,Lin C H.Talanta,2007,72(2):368 |
| [101] | Kitagawa S,Noji A,Matsushita N,et al.Electrophoresis,2011,32(12):1480 |
| [102] | Zhang L,Xu Y,Li Y P.Chinese Journal of Chromatography(张良,许杨,李燕萍.色谱),2010,28(4):393 |
| [103] | Gan N,Li T H,Wang L Y,et al.Chinese Journal of Chromatography(干宁,李天华,王鲁雁,等.色谱),2007,25(6):934 |
| [104] | Gong M,Wehmeyer K R,Limbach P A.J Chromatogr A,2006,1125 (2):263 |
| [105] | Zhang X,Zhang Z.J Pharm Biomed Anal,2011,56(2):330 |
| [106] | Gong M,Wehmeyer K R,Limbach P A,et al. Anal Chem,2006,78(17):6035 |
| [107] | Quirino J,Terabe S.Anal Chem,2000,72(6):1023 |
| [108] | Fang N,Meng P,Zhang H,et al.Analyst,2007,132 (2):127 |
| [109] | Maijo I,Borrull F,Aguilar C,et al.Chromatographia,2011,73(1/2):83 |
| [110] | Cheng H L,Jong Y J,Li J H,et al.Electrophoresis,2006,27(23):4711 |
| [111] | Huang H Y,Hsieh S H.J Chromatogr A,2007,1164(1/2):313 |
| [112] | Yang Y,Nie H,Li C,et al.Talanta,2010,82(5):1797 |
| [113] | Huang H Y,Lien W C,Huang I Y.Electrophoresis,2006,27(16):3202 |
| [114] | Li X,Hu J,Han H.J Sep Sci,2011,34(3):323 |
| [115] | Lin Y H,Li J H,Ko W K,et al.J Chromatogr A,2006,1130(2):281 |
| [116] | Lin Y H,Lee M R,Lee R J,et al.J Chromatogr A,2007,1145(1/2):234 |
| [117] | Lin Y H,Chiang J F,Lee M R,et al.Electrophoresis,2008,29(11):2340 |
| [118] | Chen X,Yuan H P,Cao Y H,et al.Chinese Journal of Chromatography(陈新,袁红萍,曹玉华,等.色谱),2010,28(9):889 |
| [119] | Valtcheva L,Mohammad J,Pettersson G,et al.J Chromatogr A,1993,638(2):263 |
| [120] | Sueyoshi K,Kitagawa F,Otsuka K.Anal Chem,2008,80(4):1255 |
| [121] | Quirino J.Electrophoresis,2011,32(6/7):665 |
| [122] | Ge L,Yong J W H,Tan S N,et al.Electrophoresis,2008,29(10):2126 |
| [123] | Ge L,Tan S N,Yong J W H,et al. Electrophoresis,2008,29(10):2024 |
| [124] | Huang J,Kang J.J Chromatogr B,2007,846(1/2):364 |
| [125] | Quirino J.J Chromatogr A,2009,1216(2):294 |
| [126] | Guidote A,Quirino J.J Chromatogr A,2010,1217(40):6290 |
| [127] | Quirino J.J Chromatogr A,2010,1217(49):7776 |
| [128] | Quirino J,Guidote A.J Chromatogr A,2011,1218(7):1004 |
| [129] | Quirino J,Haddad P.Anal Chem,2008,80(17):6824 |
| [130] | Quirino J P,Haddad P R. Electrophoresis,2009,30(10):1670 |
| [131] | Britz-McKibbin P,Otsuka K,Terabe S.Anal Chem,2002,74(15):3736 |
| [132] | Zhu Z,Zhou X,Yan N,et al.J Chromatogr A,2010,1217(11):1856 |
| [133] | Zhang H,Zhou L,Chen X. Electrophoresis,2008,29(7):1556 |
| [134] | Jin Y,Meng L C,Li M X,et al.Electrophoresis,2010,31(23/24):3913 |
| [135] | Cheng H,Chiou S,Liao Y,et al.Anal Bioanal Chem,2010,398(5):2183 |
| [136] | Luo X,Jiang X,Tu X,et al.Electrophoresis,2010,31(4):688 |
| [137] | Zhang Z,Du X,Li X.Anal Chem,2011,83(4):1291 |
| [138] | Zhang X W,Zhang Z X.Chinese Journal of Chromatography(张效伟,张召香.色谱),2010,28(4):397 |
| [139] | Beckers J,Bo(c)ek P. Electrophoresis,2000,21 (14):2747 |
| [140] | Urbánek M,K(r)ivánková L,Bo(c)ek P. Electrophoresis,2003,24 (3):466 |
| [141] | Timerbaev A,Hirokawa T. Electrophoresis,2006,27(1):323 |
| [142] | K(r)ivánková L,Pant(u)(c)ková P,Gebauer P,et al.Electrophoresis,2003,24(3):505 |
| [143] | Petr J,Maier V,Horakova J,et al.J Sep Sci,2006,29(18):2705 |
| [144] | Jung B,Bharadwaj R,Santiago J.Anal Chem,2006,78(7):2319 |
| [145] | Yan N,Zhu Z,Ding N,et al.J ChromatogrA,2009,1216(49):8665 |
| [146] | Breadmore M.Electrophoresis,2008,29(5):1082 |
| [147] | Shackman J,Ross D.Anal Chem,2007,79(17):6641 |
| [148] | Mamunoorn M,Jenkins R J,Davis N I,et al.J Chromatogr A,2008,1202(2):203 |
| [149] | Davis N I,Mamunooru M,Vyas C A,et al.Anal Chem,2009,81(13):5452 |
| [150] | Vyas C A,Mamunooru M,Shackman J G. Chromatographia,2009,70(1/2):151 |
| [151] | Hirokawa T,Okamoto H,Ga(s) B.Electrophoresis,2003,24(3):498 |
| [152] | Dawod M,Breadmore M,Guijt R,et al.J Chromatogr A,2009,1216(15):3380 |
| [153] | Xu Z Q,Hirokawa T.Chinese Journal of Chromatography (徐中其,广川健.色谱),2009,27(1):102 |
| [154] | Xu Z,Nakamura K,Timerbaev A,et al.Anal Chem,2011,83(1):398 |
| [155] | Xu Z,Kawahito K,Ye X,et al.Electrophoresis,2011,32(10):1193 |
| [156] | Xu Z,Murata K,Arai A,et al. Biomicrofluidics,doi:10.1063/1.3366719 |
| [157] | Lu Y,Breadmore M C.J Chromatogr A,2010,1217(46):7282 |
| [158] | Hirokawa T,Takayama Y,Arai A,et al.Electrophoresis,2008,29 (9):1829 |
| [159] | Busnel J M,Descroix S,Godfrin D,et al.Electrophoresis,2006,27(18):3591 |
| [160] | Bottenus D,Jubery T,Ouyang Y,etal.Lab Chip,2011,11(5):890 |
| [161] | Bahga S,Kaigala G,Bercovici M,et al.Electrophoresis,2011,32(5):563 |
| [162] | Shihabi Z.Electrophoresis,2000,21(14):2872 |
| [163] | Shihabi Z.Electrophoresis,2002,23(11):1612 |
| [164] | Chen Y,Xu L,Zhang L,et al.Anal Biochem,2008,380(2):297 |
| [165] | Botello I,Borrull F,Calull M,et al.Anal Bioanal Chem,2011,400(2):527 |
| [166] | Wang H L,Lu M L,Le X C.Anal Chem,2005,77(15):4985 |
| [167] | Wang Z X,Lu M L,Wang X L,et al.Anal Chem,2009,81(24):10285 |
| [168] | Wang X L,Song Y L,Song M Y,et al.Anal Chem,2009,81(18):7885 |
| [169] | Wang Z X,Wang C,Yin J F,et al. Electrophoresis,2008,29 (22):4454 |
| [170] | Wang J,Zhang Y,Okamoto Y,et al.Analyst,2011,136(6):1142 |
| [171] | Persat A,Marshall L A,Santiago J G.Anal Chem,2009,81(22):9507 |
| [172] | Xu Z,Esumi T,Ikuta N,et al.J Chromatogr A,2009,1216(17):3602 |
| [173] | Liu D,Ou Z,Xu M,et al.J Chromatogr A,2008,1214(1/2):165 |
| [174] | Liu D,Shi M,Huang H,et al.J Chromatogr B,2006,844(1):32 |
| [175] | Persat A,Chivukula R,Mendell J,et al.Anal Chem,2010,82(23):9631 |
| [176] | Schoch R B,Ronaghi M,Santiago J G.Lab Chip,2009,9(15):2145 |
| [177] | Persat A,Santiago J.Anal Chem,2011,83(6):2310 |
| [178] | Bercovici M,Kalgala G,Mach K,et al.Anal Chem,2011,83(11):4110 |
| [179] | Wang Y,Wang H,Ju S,et al.Labmedicine,2009,40(9):544 |
| [180] | Medina-Casanellas S,Benavente F,Barbosa J,et al.Electrophoresis,2011,32(13):1750 |
| [181] | Xia S,Zhang L,Qiu B,et al.Rapid Commun Mass Spectrom,2008,22(23):3719 |
| [182] | Gupta R,Baldock S J,Fielden P R,et al.J Chromatogr A,2010,1217(51):8026 |
| [183] | Ranc V,Stanova A,Marak J,et al.J Chromatogr A,2011,1218(2):205 |
| [184] | Marak J,Stanova A,Gajdostinova S,et al.Electrophoresis,2011,32(11):1273 |
| [185] | Pantuckova P,Krivankova L.Electrophoresis,2010,31(20):3391 |
| [186] | Mikus P,Marakova K,Marak J,et al.J Chromatogr B,2008,875 (1):266 |
| [187] | Zheng L,Zhang L,Tong P,et al.Talanta,2010,81 (4/5):1288 |
| [188] | Novakova M,Krivankova L.Electrophoresis,2008,29(8):1694 |
| [189] | Knob R,Marak J,Stanova A,et al.J Chromatogr A,2010,1217(20):3446 |
| [190] | Kosobucki P,Buszewski B.Anal Lett,2010,43(16):2631 |
| [191] | Quirino J.JSep Sci,2011,34(9):1020 |
| [192] | Huang L,Lin Q,Chen Y,et al.Anal Methods,2011,3(2):294 |
| [193] | Zhang Z X,Zhang X W,Wang J J,et al.Anal Bioanal Chem,2008,390(6):1645 |
| [194] | Zhang Z,Zhang X,Li F. Science China:Chemistry,2010,53(5):1183 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%