淫羊藿甙和朝藿定A、B、C是淫羊藿中重要的活性成分,本研究应用工业制备高效液相色谱从淫羊藿粗提物中分离制备了这4个成分.淫羊藿粗提物经大孔吸附树脂粗分离获得相应的组分后,利用工业制备高效液相色谱完成精制纯化.采用自装填Chromatorex C18制备柱(220mm×77 mm,10 μm),乙腈-水(26:74或30:70,v/v)为流动相进行洗脱,在35 min内,实现了这4种成分的基线分离及规模制备.从300 g粗提物(总黄酮含量约20%)中获得淫羊藿甙33 g、朝藿定C4.6g、朝藿定B3.7g和朝藿定A0.6g,产品纯度均达到98%以上.此方法通过两步分离即可实现这4种成分的完全分离,具有快速高效、产品纯度高的特点,适于淫羊藿中淫羊藿甙、朝藿定A、B、C系列对照品的规模制备.
参考文献
[1] | Department of Health of Hong Kong Special Administrative Region. Hong Kong Chinese Material Medica Standards.Hong Kong:Department of Health of Hongkong Special Administrative Region(香港特别行政区卫生署.香港中药材标准.香港:香港特别行政区卫生署),2005 |
[2] | Pharmacopoeia Commission of People' s Republic of China.Pharmacopoeia of People' s Republic of China:Part Ⅰ.Beijing:China Medical Science Press(国家药典委员会.中华人民共和国药典:一部.北京:中国医药科技出版社),2010 |
[3] | Guo B L,Yu J G,Xiao P G.China Journal of Chinese Materia Medica(郭宝林,余竞光,肖培根.中国中药杂志),1996,21(6):353 |
[4] | Li W K,Xiao P G,Pan J Q,et al.Chinese Pharmaceutical Journal(李文魁,肖培根,潘景岐,等.中国药学杂志),1996,31(6):332 |
[5] | Liu R M,Li A F,Sun A L,et al.J Chromatogr A,2005,1064:53 |
[6] | Hu Y F,Zhu S(胡云峰,朱升).CN,200510061251.1. 2009-07-08 |
[7] | Wang X C,Zhou N,Feng Z X,et al(王晓春,周宁,冯泽熹,等). CN, 200610201222.5. 2008-10-01 |
[8] | Wang C Z,Geng X D.Chinese Journal of Analytical Chemistry(王超展,耿信笃.分析化学),2005,33(1):106 |
[9] | Guo B L,Bian Q Y,Huang W H,et al(郭宝林,卞庆亚,黄文华,等).CN,200710002610.5.2007-07-18 |
[10] | Zhang X Z.[PhD Dissertation].Dalian:Dalian Institute of Chemical Physics,Chinese Academy of Sciences(张晓哲.[博士学位论文].大连:中国科学院大连化学物理研究所),2004 |
[11] | Hostettman K,Marston A,Hostettman M. Preparative Chromatography Techniques. Zhao W M, Zhang T Y,transl.Beijing:Science Press(霍夫泰特曼K,马斯顿A,霍夫泰特曼M.制备色谱技术.赵维民,张天佑,译.北京:科学出版社),2000:83 |
[12] | Majors R E.LCGC Asia Pacific,2004,7(3):8 |
[13] | Gao M Z,Yuan X Y,Xiao H B.Chinese Journal of Chromatography(高明哲,袁晓艳,肖红斌.色谱),2008,26(3):362 |
[14] | Wang L X,Gao M Z,Xiao H B.Chinese Journal of Chromatography(王龙星,高明哲,肖红斌.色谱),2008,26(4):523 |
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