建立了一种同时测定布洛芬注射液中布洛芬和精氨酸含量的超高效液相色谱方法.精氨酸与衍生化试剂2,4-二硝基氟苯(DNFB)反应后,与布洛芬同时在超高效液相色谱-二极管阵列检测器(UPLC-PDA)上检测.采用BEH C18色谱柱(50 mm×2.1 mm,1.7μm),以乙腈-0.05 mol/L磷酸二氢钾缓冲液(pH 2.5)为流动相进行梯度洗脱,流速为0.4mL/min,柱温为30℃,检测波长分别为357 nm(精氨酸衍生物)和220 nm(布洛芬).结果表明,布洛芬与精氨酸分别在2.0 ～ 100.5 mg/L和1.7～84.5mg/L范围内呈良好的线性关系,相关系数(r)均为0.9997;平均回收率分别为99.8％和99.6％,相对标准偏差(RSDs)分别为0.37％和0.25％；定量限(信噪比(S/N)=10)分别为0.1 ng和0.2 ng;检出限(S/N=3)分别为0.03 ng和0.05 ng.本方法快速、准确,重复性好,可较全面地评价布洛芬注射液的质量.
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