基于纳米纤维的富集作用,建立了血浆中5-羟色胺(5-HT)的柱前衍生高效液相色谱-电化学检测(HPLC-ECD)分析方法.用10%(v/v)高氯酸溶液沉淀血浆蛋白,离心后取上清液,用0.1 mol/L 的四苯硼酸钠溶液调节pH值至8.5,加入衍生剂邻苯二甲醛溶液于30 ℃衍生4 min,经纳米纤维固相萃取柱净化富集后,以pH 5.4的0.05 mol/L 磷酸二氢钾缓冲液(含0.25 mmol/L 乙二胺四乙酸)-甲醇(60∶40,v/v)为流动相在C18色谱柱(150 mm×4.6 mm,5 μm)上进行分离,进样量为20 μL.结果表明,在高、中、低3个加标水平下,血浆中5-HT的加标回收率为95.6% ～101.4%,日内与日间相对标准偏差均小于5%(n=3).方法的定量线性范围为5～500 μg/L,检出限为1 μg/L.该方法操作简单、快速,具有较高的灵敏度和较高的重现性,能够用于血浆中5-HT的含量测定.
参考文献
| [1] | Goyal R N,Gupta V K,Oyama M,et al.Talanta,2007,72:976 |
| [2] | Kema I P,De Vries E G,Muskiet F A J.J Chromatogr B,2000,747:33 |
| [3] | Siever L J,Kahn R S,Lawlor B A,et al.Pharmacol Rev,1991,43:509 |
| [4] | Seibold J R.J Cardiovasc Pharmacol,1985,7:S95 |
| [5] | Jackson B P,Dietz S M,Wightman R M.Anal Chem,1995,67:1115 |
| [6] | Chang C W,Tseng W L.Anal Chem,2010,82:2696 |
| [7] | Zhao Y Y,Liu L Y,Han Y Y,et al.Chinese Journal of Chromatography (赵燕燕,刘丽艳,韩媛媛,等.色谱),2011,29(2):146 |
| [8] | Zhao H Y,Duan C L,Fan C X,et al.Chinese Journal of Analytical Chemistry (赵焕英,段春礼,范春香,等.分析化学),2009,37(3):330 |
| [9] | Pucci V,Ferranti A,Mandrioli R,et al.Anal Chim Acta,2003,488:97 |
| [10] | Pobozy E,Michalski A,Sotowska-Brochocka J,et al.J Sep Sci,2005,28:2165 |
| [11] | Peterson Z D,Lee M L,Graves S W.J Chromatogr B,2004,810:101 |
| [12] | Li M D,Tseng W L,Cheng T L.J Chromatogr A,2009,1216:6451 |
| [13] | Liu Z Y,Kang X J,Fang F.Microchim Acta,2010,168:59 |
| [14] | Qi D J,Kang X J,Chen L Q,et al.Anal Bioanal Chem,2008,390(3):929 |
| [15] | Tcherkas Y V,Kartsova L A,Krasnova I N.J Chromatogr A,2001,913:303 |
| [16] | Wu J H,Zheng M M,Feng N Z.The Eleventh Annual Analytical Chemistry Meeting of Chemistry and Chemical Industry Society of Hubei (吴剑虹,郑明明,冯钮铸.湖北省化学化工学会第十一届分析化学专业年会),2007:113 |
| [17] | Li X Q,Li Y J,Wang Y.Practical Pharmacy and Clinical Remedies (李雪芹,李运景,王勇.实用药物与临床),2010,13(2):119 |
| [18] | Li J N,Liang G D,Zhu Q M,et al.Chinese Journal of Health Laboratory Technology (李静娜,梁高道,朱其明,等.中国卫生检验杂志),2009,19(8):1722 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%