以L-酒石酸正己酯-硼酸配合物为手性流动相添加剂,建立了普萘洛尔、艾司洛尔、美托洛尔、比索洛尔和索他洛尔5种β-受体阻滞剂的反相高效液相色谱手性分离方法.对影响对映体分离的主要因素:L-酒石酸正己酯和硼酸的浓度,缓冲溶液的种类、浓度、pH值,有机改性剂甲醇的含量以及对映体分子结构等进行了详细考察.最佳色谱条件为:Venusil MP-C1s色谱柱(250mm×4.6mm,5μm)；流动相为15 mmol/L乙酸铵-甲醇(体积比为20∶80或30∶70(索他洛尔)),含60 mmol/L硼酸,70 mmol/L L-酒石酸正己酯,乙酸调节pH值至6.00；检测波长214nm.在最佳分离条件下,5对对映体可以分别获得基线分离.
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