以自旋标记荧光探针4-(( 9-acridinecarbonyl )amino)-2,2,6,6-tetramethylpiperidin-1-oxyl( TEMPO-9-AC)研究了卷烟主流烟气中的碳中心自由基.以吸烟机抽吸卷烟,以弱荧光的TEMPO-9-AC作为捕集剂捕集卷烟主流烟气中的碳中心自由基,生成稳定的强荧光的碳中心自由基捕集加合物,以高效液相色谱-三重四极杆串联质谱联用仪(HPLC-MS/MS)对其结构进行了确认,并建立了高效液相色谱-荧光检测(HPLC-FLD)的定量方法.结果表明,方法的检出限为0.318 nmol/cig,相对标准偏差(RSD)为3.5％～9.7％；利用TEMPO-9-AC捕集体系鉴别出了10种碳中心自由基；对5种代表性卷烟烟气中的碳中心自由基进行了定量计算,获得了它们在主流烟气中碳中心自由基的含量数据,并发现碳中心自由基总量与焦油释放量之间具有高度的相关性.本法检出限低,重复性好,适用于卷烟主流烟气中碳中心自由基的结构验证及释放量的检测分析.
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