在高效液相色谱-质谱分析指导下,针对性地分离制备了黄芩药材中系列黄酮成分对照品.首先对黄芩药材乙醇提取物进行液相色谱-质谱分析,获得各色谱峰的保留时间、紫外光谱和质谱特征.经波谱数据解析结合文献对比,鉴定了黄芩药材中的19种黄酮类成分.然后根据液相色谱-质谱分析结果和文献,设计了目标成分对照品的制备流程,采用低压制备柱色谱法依次制备了黄芩苷、汉黄芩苷、黄芩素、汉黄芩素和千层纸素A共5种黄酮类成分的对照品.结果表明这5种黄酮类成分对照品的纯度均大于98％.该方法可用于针对性地快速分离制备中药中的化学成分.
参考文献
| [1] | Pharmacopoeia Commission of People's Republic of China.Pharmacopoeia of People's Republic of China.Part 1.Beijing:China Medical Science Press(国家药典委员会.中华人民共和国药典.一部.北京:中国医药出版社),2010:282 |
| [2] | Wen H Z,Xiao S Y,Wang Y M,et al.Natural Product Research and Development(温华珍,肖盛元,王义明,等.天然产物研究与开发),2004,16(6):575 |
| [3] | Huang L Q.Study on the Quality Standards of Traditional Chinese Medicine.Beijing:People's Medical Publishing House(黄璐琦.中药材质量标准研究.北京:人民卫生出版社),2006:571 |
| [4] | Zhang J,Xiao L H,Li F M.Journal of Chinese Medicinal Materials(张箭,肖丽和,李发美.中药材),2005,28(5):389 |
| [5] | Jin Y,Xiao S S,Sun Y Q.Chinese Journal of Chromatography(金郁,肖珊珊,孙毓庆.色谱),2003,21(6):558 |
| [6] | Gao T,Hu Q,Panz Q.Chemistry and Bioengineering(高婷,胡倩,潘志权.化学与生物工程),2011,28(5):89 |
| [7] | Bai J,Wang D M,Yang D P.Journal of Instrumental Analysis(白洁,王冬梅,杨得坡.分析测试学报),2008,27(2):185 |
| [8] | Han J,Ye M,Xu M,et al.J Chromatogr B,2007,848:355 |
| [9] | Liu G Z,Ma J Y,Chen Y Z,et al.JChromatogr A,2009,1216:4809 |
| [10] | Zhang L,Zhang R W,Li Q,et al.Chromatographia,2007,66:13 |
| [11] | Wu W,Yan C Y,LiL,et al.JChromatogrA,2004,1047:213 |
| [12] | Liu Y X.[MS Dissertation].Shenyang:Shenyang Pharmaceutical University(刘英学.[硕士学位论文].沈阳:沈阳药科大学),2008 |
| [13] | Zhou X Q,Liang H,Lu X H,et al.Journal of Peking University:Health Sciences(周锡钦,梁鸿,路新华,等北京大学学报:医学版),2009,41(5):578 |
| [14] | Wang W.[MS Dissertation].Beijing:Beijing University of Chinese Medicine(王微.[硕士学位论文].北京:北京中医药大学),2009 |
| [15] | Wang H Y.[PhD Dissertation].Shenyang:Shenyang Pharmaceutical University(王红燕.[博士学位论文].沈阳:沈阳药科大学),2002 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%