建立了超高效液相色谱-串联质谱法(UPLC-MS/MS)测定脐橙中的橘红2号染料.样品用乙腈提取,NH2固相萃取小柱进行净化后,用超高效液相色谱-串联质谱仪进行测定,使用Waters Acquity UPLC BEH C18柱(50 mm×2.1 mm,1.7 μm)进行分离,以水和乙腈作为流动相梯度洗脱,流速为0.3 mL/min.质谱采用电喷雾离子源、正离子采集模式(ESI+),采集方式为多反应监测( MRM).方法的线性范围为0.1～100μg/L(线性相关系数r2=0.996 5),检出限为0.05 μg/kg,定量限为0.1μg/kg.在空白脐橙样品中添加1.0、10.0和100.0 μg/kg的橘红2号染料,其回收率为84.2％ ～ 94.0％,相对标准偏差为2.86％ ～10.15％.利用该方法对市场上随机采集的18份脐橙样品进行了检测,结果显示,从2份样品中检出橘红2号染料.该方法灵敏度高,准确度好,适用于对脐橙中橘红2号染料的检测.
参考文献
| [1] | Shui Q.Jiangsu Agricultural Science and Technology News (水清.江苏农业科技报),2008-01-05 (7).http://home.jaas.ac.cn/department/jaasnic/200508/172.html |
| [2] | U.S.Food and Drug Administration.Code of Federal Regulations Title 21.(2011-04-01)[2012-03-01].http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfCFR/CFRSearch.cfm? fr =74.302 |
| [3] | Wikipedia:Citrus Red 2.[2012-03-01].http://en.wikipedia.org/wiki/E 121 |
| [4] | Ge Y.Quality and Standardization(葛宇.质量与标准化),2011(9):31 |
| [5] | FAO Nutrition Meetings Report Series No.46A WHO/FOOD ADD/70.36.World Health Organization and International Programme on Chemical Safety.[2012-03-01].http://www.inchem.org/documents/jecfa/jecmono/v46aje10.htm |
| [6] | Hope C,Connors R.J Assoc Off Anal Chem,1989,72(5):705 |
| [7] | Shanghai ANPEL Scientific Instrument Co.,Ltd.Determination of Citrus Red2 in Citrus and Juice by SPE-HPLC.(上海安谱科学仪器有限公司.柑橘和果汁中柑桔红2号染料的检测(SPE-HPLC)).[2012-03-01].http://wenku.baidu.com/view/224f3ad184254b35eefd3444.html |
| [8] | Zhao S,Zhang J,Yang Y,et al.Chinese Journal of Chromatography(赵珊,张晶,杨奕,等.色谱),2010,28(4):356 |
| [9] | Hu X,Xiao G,Pan W,et al.Chinese Journal of Chromatography(胡侠,肖光,潘炜,等.色谱),2010,28(6):590 |
| [10] | Lin S J,Tu H Y,Sun L,et al.Chinese Journal of Chromatography(林赛君,屠海云,孙岚,等.色谱),2011,29(1):79 |
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