建立了复方化学消毒剂中常用有效成分醋酸洗必泰和苯扎氯铵(C12-BAC、C14-BAC及C16-BAC)同时分离测定的毛细管电泳(CE)方法.以37 cm×50 μm未涂层熔融石英毛细管为分离柱,以150 mmol/L磷酸二氢钠-62.5mmol/L磷酸(pH 2.5)缓冲液(含体积分数为40％的乙腈)为分离缓冲溶液,50 mmol/L醋酸-乙腈(体积比为1∶1)为样品介质,检测波长为214 nm.方法的日内及日间精密度分别小于3.0％及3.7％.醋酸洗必泰、C12-BAC、C14-BAC及C16-BAC的检出限(信噪比为3)分别为0.3、0.5、0.5、0.5 mg/L,定量限(信噪比为10)分别为1.0、1.5、1 5和1.5 mg/L,在1.0～ 400、1.5～ 200、1.5 ～ 200和1.5 ～ 200 mg/L范围内,4种有效成分的校正峰面积与相应质量浓度均具有良好的线性关系,相关系数分别为0.999 5、0.999 8、0.999 7和0.999 8.加标回收率为93.83％～ 104.97％.将该法用于实际样品分析,并与液相色谱的分析结果进行比对,获得满意结果.
参考文献
| [1] | Yang H M,Yi B.Modern Hospital Disinfection.Beijing:People's Military Medical Publishing House(杨华明,易滨.现代医院消毒学.北京:人民军医出版社),2002:138 |
| [2] | The United States Pharmacopeial Convention.The United States Pharmacopeia/National Formulary,USP 32-NF 27,2009:1174 |
| [3] | Ding X J,Che Y P,Zhao H Y,et al.Journal of Instrumental Analysis(丁晓静,车宜平,赵海燕,等.分析测试学报),2006,25(6):63 |
| [4] | Ma L H,Wang H G,Li F Y,et al.Modern Medicine&Health(马丽红,王洪刚,李凤云,等.现代医药卫生),2010,26(12):1778 |
| [5] | The Ministry of Health Justice and Supervision Division.Technical Standard for Disinfection (2002 ed). Beijing:Ministry of Health of the People's Republic of China(卫生部卫生司法与监督司.消毒技术规范(2002版)北京:中华人民共和国卫生部),2002:116 |
| [6] | Liu J Q,Zhang X B,Yang R C,et al.Chinese Journal of Disinfection(刘吉起,张向兵,杨瑞春,等.中国消毒学杂志),2010,27(6):682 |
| [7] | Liu Y Q,Wang H,Yang H M,et al. Chinese Journal of Chromatography(刘艳琴,王浩,杨红梅,等色谱),2011,29(5):458 |
| [8] | Labranche L P,Dumont S N,Levesque S,et al. J Pharm Biomed Anal,2007,43:989 |
| [9] | Zhao J,Fan Y F.China Medical Herald(赵静,范义凤 中国医药导报),2008,5(31):27 |
| [10] | Wang Z S,Wu S M.China Pharmaceuticals(王琤帅,吴韶铭.中国药业),2010,19(4):19 |
| [11] | Zhang Y P,Chen Q Z,LaiX M.China Modern Medicine (张云平,陈启钊,赖秀梅.中国当代医药),2010,17(14):41 |
| [12] | Lu S G,Liu H L,Yang R C.Chinese Journal of Disinfection(卢素格,刘红丽,杨瑞春.中国消毒学杂志),2011,28(3):317 |
| [13] | Havlikova L,Matysova L,Novakova L,et al.J Pharm Biomed Anal,2007,43:1169 |
| [14] | XueY,TangM,Hieda Y,etal.J AnalToxicol,2009,33:85 |
| [15] | Hou Y H,Wu C Y,Ding W H.J Chromatogr A,2002,976:207 |
| [16] | Ding X J,Zhao R,Zhao S,et al.Chinese Journal of Chromatography(丁晓静,赵榕,赵珊,等.色谱),2003,21(5):520 |
| [17] | Ding X J,Mou S F,Zhao S.J Chromatogr A,2004,1039:209 |
| [18] | Zou X L,Li Y Q,Mao H X,et al.Chinese Journal of Chromatography(邹晓莉,黎源倩,毛红霞,等.色谱),2005,23(3):264 |
| [19] | Abad-Villar E M,Etter S F,Thiel M A,et al.Anal Chim Acta,2006,561:133 |
| [20] | Altria K.LCGC Asia Pacific,2007,10(2):25 |
| [21] | Ding X J,Yang Y Y,Li Y,et al.Physical Testing and Chemical Analysis Part B:Chemical Analysis(丁晓静,杨媛媛,李芸,等.理化检验:化学分册),2010,46(12):1369 |
| [22] | Wang Q,Ding X J,Wang X Y,et al.Chinese Journal of Analytical Chemistry(王茜,丁晓静,王心宇,等.分析化学),2006,34(8):1161 |
| [23] | Wang P,Ding X J,Li Y,et al.J AOAC Int,2012,95(4).DOI:10.5740/jaoacint.11-043 |
| [24] | Mou S F,Liu K N.The Method and Application of Ion Chromatography.Beijing:Chemical Industry Press(牟世芬,刘克纳.离子色谱方法及应用,北京:化学工业出版社),2000:173 |
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