建立了一种固相分散萃取-超高效液相色谱-串联质谱(QuEChERS-UPLC-MS/MS)同时检测火锅食材中11种喹诺酮类药物的方法 样品用5％甲酸乙腈溶液提取后加入盐析剂分层,提取液中加入C18和PSA填料进行基质分散固相净化,浓缩后经Poroshell 120 EC-C18柱分离,用电喷雾离子源正离子多反应监测(MRM)模式串联质谱进行检测 11种药物在1.0～ 100.0 μg/kg范围内具有较好的线性关系,相关系数均大于0.998.该方法检出限(LOD)为1.8 ～3.1 μg/kg,定量限(LOQ)为6.0～10.3 μg/kg.11种药物的回收率为70.1％～ 100.3％,相对标准偏差(RSD)为2.42％ ～ 10.88％.该方法简便快速、灵敏度高、准确度好、使用范围广,可作为火锅食材中11种喹诺酮类药物残留的确证方法.
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