建立了用于测定瓜果中多效唑、氯吡脲、异戊烯腺嘌呤和6-苄氨基嘌呤等4种植物生长调节剂残留量的高效液相色谱-串联质谱分析方法.样品用乙腈提取,经阳离子交换固相萃取柱净化后,用Agilent XDB-C18色谱柱分离,以5 mmol/L乙酸铵溶液和含0.1％(体积分数)甲酸的乙腈溶液为流动相进行梯度洗脱.质谱采用电喷雾正离子(ESI+)模式电离,多反应监测(MRM)模式检测,基质匹配标准溶液外标法定量.4种植物生长调节剂在各自的范围内线性很好,相关系数均大于0.999.选取了黄瓜和苹果作为代表性基质进行添加回收试验,定量限(信噪比大于l0)在0.04 ～1.35 μg/kg之间,检出限(信噪比大于3)在0.01～0.41 μg/kg之间,在3个添加水平下回收率范围为81.0％～93.3％,相对标准偏差(RSDs)为3.5％ ～ 9.5％.该方法的灵敏度、准确度和精密度均符合农药残留测定的技术要求,适用于瓜果中这4种植物生长调节剂残留量的检测.
参考文献
| [1] | Song Y,Zhang Y H,Huang X,et al.Chinese Journal of Analytical Chemistry(宋莹,张耀海,黄霞,等.分析化学),2011,39(8):1270 |
| [2] | Zhou Y F,Zhang S R.Pesticides(周永丰,张寿儒.农药), 2003,42(11):43 |
| [3] | ZhaoDY,LiM,YuJL,et al.Pesticides(赵德友,李美,于建垒,等.农药),2000,39(12):39 |
| [4] | Zhang J,Wang L L,Liu H Y,et al.Chinese Journal of Analytical Chemistry(张洁,王琳琳,刘海燕,等.分析化学), 2010,38(1):147 |
| [5] | Jin M C,Chen X H,Li X P,et al.Analytical Instrumentation(金米聪,陈晓红,李小平,等.分析仪器),2005(3): 29 |
| [6] | WTO/TBT-SPS Notification and Enquiry of China.Maximum Residue Limits Inquiry(中华人民共和国WTO/TBTSPS国家通报咨询中心.最大残留限量查询).(2012-08-20)[2012-12-02].http:∥www.tbt-sps,gov.cn/jpmrl/MRLS/ Pages/MRLSsearch.aspx |
| [7] | Editorial Committee of Maximum Residue Limits of Chemical Residues in Food.Maximum Residue Limits of Chemical Residues in Food.Beijing:China Standards Press(《食品中农业化学品残留限量》编委会.食品中农业化学品残留限量.北京:中国标准出版社),2006:44-47,53-62,684-685,700-703,937 |
| [8] | United States Department of Agriculture(FAS).Pesticide MRL Database (frequently).[2012-12-02].http:∥www.mrldatabase,com |
| [9] | GB 2760-1996 |
| [10] | Qi M F.Jiangsu Agricultural Sciences(齐明芳.江苏农业科学),2011,39(6):486 |
| [11] | Lu Y M,Yi G B,Chen C B,et al.Journal of Instrumental Analysis(陆益民,易国斌,陈创彬,等.分析测试学报),2011,30(2):186 |
| [12] | Wang W.Agrochemicals(汪伟.农药),2009,48(2):116 |
| [13] | Xia M,An L T,Yi Q F,et al.Chemical Reagents(夏敏,安礼涛,尹起范,等.化学试剂),2009,31(11):913 |
| [14] | Xu Y,Shou L F,Yu M,et al.Chinese Journal of Pesticide Science(徐永,寿林飞,虞淼,等.农药学学报),2012,14 (1):6l |
| [15] | Kaushik B,Dasharath P O,Soma D,et al.J Chromatogr A,2007,1173(1/2):98 |
| [16] | Yu H,Lu Y,Wang J J,et al.Xinjiang Agricultural Sciences (于红,鹿毅,王静静,等.新疆农业科学),2011,48(1):187 |
| [17] | Camino-Sánchez F J,Zafra-Gómez A,Ruiz-García J,et al.J Food Compos Anal,2011,24(3):427 |
| [18] | Lü Y,Zhao J,Yang T,et al.China Measurement＆Testing Technology(吕燕,赵健,杨挺,等.中国测试),2010,36 (4):57 |
| [19] | Hogenboom A C,Hofman M P,Kok S J,et al.J Chromatogr A,2000,892(1/2):379 |
| [20] | Hu J T,Sheng Y,Fang Z,et al.Chinese Journal of Chromatography(胡江涛,盛毅,方智,等.色谱),2007,25 (3):441 |
| [21] | Liu J J,Gong P,Zhang X M,et al.Chinese Journal of Chromatography(刘靖靖,宫萍,张晓梅,等.色谱),2012,30(10):1012 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%