建立了高效液相色谱-串联质谱法（ HPLC-MS/MS）用于测定辽东！木不同部位中的！木皂苷Ⅱ、！木皂苷Ⅳ、！木皂苷Ⅴ、！木皂苷Ⅹ和！木叶皂苷Ⅱ的含量，并比较了！木不同部位中上述5种皂苷的含量差异。以Allti-ma C18柱（250 mm×4.6 mm，5μm）为分析柱，以乙腈和0.1％（体积分数）甲酸水溶液为流动相，梯度洗脱，流速为0.8 mL/min。在电喷雾正离子多反应监测（ MRM）模式下进行检测。结果表明该方法中！木皂苷Ⅱ、！木皂苷Ⅳ、！木皂苷Ⅴ、！木皂苷Ⅹ和！木叶皂苷Ⅱ分别在0.17~108μg/L、0.53~329μg/L、0.77~480μg/L、0.77~480μg/L和0.82~510μg/L范围内线性关系良好，相关系数（ r2）均大于0.999，提取回收率为99.0％~100.2％。该方法简单、快速、灵敏，可用于测定辽东！木药材不同部位中的皂苷类成分的含量。测定结果发现！木不同部位中上述5种皂苷总量多少的顺序为根皮﹥叶﹥种子﹥芽。
A method was developed for the simultaneous determination of congmunosideⅡ, congmunoside Ⅳ,congmunosideⅤ,congmunosideⅩ and congmuyenosideⅡ in different parts of Aralia elata by high performance liquid chromatography-tandem mass spectrometry( HPLC-MS/MS). The chromatographic separation was performed on an Alltima C 18 analytical column (250 mm×4. 6 mm,5 μm)using the mobile phases of acetonitrile and 0. 1%(v/v)formic acid aqueous solution with gradient elution. The separated compounds were detected in multiple reaction monitoring( MRM)mode via positive electrospray ionization( ESI+). The linear ranges of congmunosideⅡ,congmunoside Ⅳ,congmunosideⅤ,congmunosideⅩ and congmuyeno-sideⅡ were 0. 17-108 μg/L,0. 53-329 μg/L,0. 77-480 μg/L,0. 77-480 μg/L and 0. 82-510μg/L,respectively. The extraction recoveries varied from 99. 0% to 100. 2%. The developed method is rapid,accurate and reproducible,and is successfully utilized as a quality control method for Aralia elata. The results indicate that the order of contents of these five saponins in different parts of Aralia elata is root bark﹥leaves﹥seeds﹥buds.
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