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采用中间体法，先将大黄素配体与γ-[（2，3）-环氧丙氧]丙基三甲氧基硅烷（KH-560）偶联剂反应制备含配体的硅氧烷试剂，然后再与硅胶键合，最终制得大黄素键合硅胶液相色谱固定相（以下简称 ESP）。通过红外光谱、元素分析和热重分析表征固定相的结构。以萘作为溶质探针，甲醇-水（60：40，v / v）为流动相，流速为0.8 mL / min，测得 ESP 柱的柱效。采用传统的反相 C18和苯基柱作参比，将 ESP 应用于系列中性、碱性和酸性芳香族化合物以及实际样品风油精的分离分析，并探讨相关的色谱分离机理。结果表明，配体大黄素被成功地键合到球形硅胶表面，测得配体键合量为0.23 mmol / g，ESP 柱理论塔板数约为19874 N / m。ESP 的偶联剂链和蒽醌环提供了疏水性的结构基础，大黄素配体还能为溶质提供π-π或 p-π、电荷转移、氢键、偶极-偶极等作用点。多位点的协同作用使得 ESP 柱具有独特和优秀的色谱分离选择性，并且无需调节 pH 值，采用简单而廉价的甲醇-水流动相就能实现胺类、酚类等极性样品的基线分离，实验条件简单、方便。

An emodin-bonded silica gel stationary phase( ESP)for high performance liquid chromatography has been synthesized by the intermediate method. The preparation process was as follows:γ-glycidoxypropyltrimethoxy silane( KH-560)firstly reacted with emodin ligand;then the intermediate was chemically immobilized to the surface of silica gel. Characterization of ESP was carried out with Fourier transform infrared spectroscopy ( FTIR ), elemental analysis and thermogravimetric analysis. Naphthalene was used as a probe to determine the col-umn efficiency with methanol-water(60 :40,v / v)as binary mobile phase at a flow rate of 0. 8 mL / min. In order to elucidate the related separation mechanism,ESP was used to separate a series of neutral,basic and acidic aromatic compounds,as well as a sample of wind medicated oil. The conventional C 18 column and phenyl column were also tested under the same chromato-graphic conditions for comparison. The results showed that the emodin ligand was successfully bonded to the surface of spherical silica gel with a 0. 23 mmol / g of bonded amount,and the theoretical plate number of ESP column was about 19 874 N / m. The coupling reagent chains and anthraquinone rings in ESP offered a structural basis for hydrophobic interaction. Mean-while,the emodin ligands provided π-π or p-π,charge transfer,hydrogen bonding,dipole-di-pole action sites for different analytes. The synergistic reaction of various action sites endowed ESP column special and excellent chromatographic separation selectivity. And a baseline sepa-ration of polar compounds such as amines and phenols on ESP was easily achieved by using common and cheap methanol-water mobile phases without buffer salts. The experimental condi-tions were simple and convenient.