亲水作用色谱（ HILIC）是替代反相色谱（ RPLC）分离强极性及亲水性化合物的另一色谱模式，其分离机理与RPLC有很大不同，具有和 RPLC互补的选择性。在 HILIC模式中，采用正相色谱（ NPLC）中的极性固定相及含高浓度有机溶剂（通常为乙腈）的水溶液为流动相。硅胶是开发最早、研究最为深入及应用最为广泛的 HILIC 固定相，本文介绍了硅胶色谱柱的 HILIC保留机理，详细概述了操作条件如硅胶柱类型、流动相组成及柱温对 HILIC 分离的影响，并对硅胶填料色谱柱的 HILIC模式的发展方向与应用前景进行了展望。
Hydrophilic interaction chromatography( HILIC)is a valuable alternative to reversed phase liquid chromatography( RPLC ) for the analysis of highly polar and hydrophilic com-pounds,in which the separation mechanism is quite different from RPLC and the separation selectivity is complementary to RPLC. This separation mode can be characterized as normal phase liquid chromatography( NPLC)on polar columns in aqueous-organic mobile phases rich in organic solvents ( usually acetonitrile ). Silica has been the earliest developed and most widely used HILIC stationary phase. This review deals with the recent advances in the develop-ment of the retention mechanism on silica column with special attention to the effects of sta-tionary phase,mobile phase composition and temperature on separation in HILIC mode. Moreo-ver,the developing trends and applications of this HILIC mode are presented.
参考文献
| [1] | Alpert A J. J Chromatogr,1990,499:177 |
| [2] | Melnikov S M,Hltzel A,Seidel-Morgenstern A,et al. Anal Chem,2013,85:8850 |
| [3] | Gama M R,da Costa Silva R G,Collins C H,et al. Trend A-nal Chem,2012,37:48 |
| [4] | Buszewski B,Noga S. Anal Bioanal Chem,2012,402:231 |
| [5] | Jandera P. Anal Chim Acta,2011,692:1 |
| [6] | Nguyen H P,Schug K A. J Sep Sci,2008,31:1465 |
| [7] | Periat A,Boccard J,Veuthey J-L,et al. J Chromatogr A, 2013,1312:49 |
| [8] | Periat A,Grand-Guillaume Perrenoud A,Guillarme D. J Sep Sci,2013,36:3141 |
| [9] | Soukup J,Janás P,Jandera P. J Chromatogr A,2013, 1286:111 |
| [10] | Louw S,Pereira A S,Lynen F,et al. J Chromatogr A, 2008,1208:90 |
| [11] | Ikegami T,Tomomatsu K,Takubo H,et al. J Chromatogr A,2008,1184:474 |
| [12] | Heaton J C,Wang X,Barber W E,et al. J Chromatogr A, 2014,1328:7 |
| [13] | Mazzarino M,Fiacco I,de la Torre X,et al. J Chromatogr A,2011,1218:8156 |
| [14] | Nemoto T,Lee X-P,Kumazawa T,et al. J Pharm Biomed Anal,2014,88:71 |
| [15] | Zhou G,Pang H,Tang Y,et al. Food Chem,2014,150:260 |
| [16] | Gokmen V,Serpen A,Mogol B A. Anal Bioanal Chem, 2012,403:2915 |
| [17] | Novkov L,Gottvald T,Vlcˇkov H,et al. J Chromatogr A,2012,1259:237 |
| [18] | Periat A,Debrus B,Rudaz S,et al. J Chromatogr A,2013, 1282:72 |
| [19] | Novkov L. J Chromatogr A,2013,1292:25 |
| [20] | Hemstrm P,Irgum K. J Sep Sci,2006,29:1784 |
| [21] | Guo Y,Gaiki S. J Chromatogr A,2011,1218:5920 |
| [22] | Li R P,Huang J X. Progress in Chemistry(李瑞萍,黄骏雄. 化学进展),2006,18(11):1508 |
| [23] | Olsen B A,Pack B W. Hydrophilic Interaction Chromatog-raphy:A Guide for Practitioners. New Jersey:John Wiley&Sons,Inc.,2013 |
| [24] | García-Gómez D,Rodríguez-Gonzalo E,Carabias-Martínez R. Trend Anal Chem,2013,47:111 |
| [25] | Naidong W. J Chromatogr B,2003,796:209 |
| [26] | Guo Y L,Yuan Q,Li R P,et al. Chinese Journal of Chro-matography(郭亚丽,袁琴,李瑞萍,等. 色谱),2012,30 (3):232 |
| [27] | Bernal J,Ares A M,Pól J,et al. J Chromatogr A,2011, 1218:7438 |
| [28] | van Nuijs A L N,Tarcomnicu I,Covaci A. J Chromatogr A, 2011,1218:5964 |
| [29] | Li R P,Guo Y L,Yuan Q. J Liq Chromatogr Rel Technol, 2011,34:1112 |
| [30] | Jian W Y,Edom R W,Xu Y,et al. J Sep Sci,2010,33:681 |
| [31] | Ares A M,Bernal J. Cent Eur J Chem,2012,10(3):534 |
| [32] | Kahsay G,Song H,Schepdael A V,et al. J Pharm Biomed Anal,2014,87:142 |
| [33] | Dejaegher B,Heyden Y V. J Sep Sci,2010,33:698 |
| [34] | Spagou K,Tsoukali H,Raikos N,et al. J Sep Sci,2010, 33:716 |
| [35] | Shen A J,Guo Z M,Liang X M. Progress in Chemistry(沈爱金,郭志谋,梁鑫淼. 化学进展),2014,26(1):10 |
| [36] | McCalley D V. J Chromatogr A,2007,1171:46 |
| [37] | EPA Method 1694:Pharmaceuticals and Personal Care Products in Water, Soil, Sediment, and Biosolids by HPLC/MS/MS. [2014-05-20]. http://water. epa. gov/scitech/methods/cwa/bioindicators/upload/2008 01 03 methods method 1694.pdf |
| [38] | Ferrer I,Zweigenbaum J A,Thurman E M,et al. J Chrom-atogr A,2010,1217:5674 |
| [39] | Soukup J,Janás P,Jandera P. J Chromatogr A,2013, 1286:111 |
| [40] | Heaton J C,Russell J J,Underwood T,et al. J Chromatogr A,2014,1347:39 |
| [41] | Pesek J J,Matyska M T,Boysen R I,et al. Trend Anal Chem,2013,42:64 |
| [42] | Quintela O, Lendoiro E, Cruz A, et al. Anal Bioanal Chem,2010,396:1703 |
| [43] | Wu X,Huang W,Lu L,et al. Anal Bioanal Chem,2010, 398:1319 |
| [44] | Peng L,Jiang T,Rong Z,et al. J Chromatogr B,2011, 879:3927 |
| [45] | Wu X,Zhu B,Lu L,et al. Food Chem,2012,133:482 |
| [46] | Luo H,Zhang L,Xue F,et al. Food Anal Methods,2014, 7:308 |
| [47] | Chauve B,Guillarme D,Cléon P,et al. J Sep Sci,2010, 33:752 |
| [48] | Grumbach E S,Diehl D M,Neue U D. J Sep Sci,2008,31:1511 |
| [49] | Orentiene A,Olsauskaite V,Vickackaite V,et al. J Chrom-atogr A,2011,1218:6884 |
| [50] | Backe W J,Yingling V,Johnson T. J Chromatogr A,2014, 1334:72 |
| [51] | Bidlingmeyer B A,Del Rios J K,Korpi J. Anal Chem, 1982,54:442 |
| [52] | Law B,Gill R,Moffat A C. J Chromatogr,1984,301:165 |
| [53] | Cox G B,Stout R W. J Chromatogr,1987,384:315 |
| [54] | McCalley D V,Neue U D. J Chromatogr A,2008,1192:225 |
| [55] | Kumar A,Heaton J C,McCalley D V. J Chromatogr A, 2013,1276:33 |
| [56] | McKeown A P,Euerby M R,Lomax H,et al. J Sep Sci, 2001,24:835 |
| [57] | Li R P,Huang J X. J Chromatogr A,2004,1041:163 |
| [58] | Li R P,Zhang Y,Lee C C,et al. J Chromatogr A,2010, 1217:1799 |
| [59] | McCalley D V. J Chromatogr A,2010,1217:3408 |
| [60] | Jin G,Guo Z,Zhang F,et al. Talanta,2008,76:522 |
| [61] | Li R P. Journal of China Three Gorges University(李瑞萍.三峡大学学报),2005,27(1):76 |
| [62] | Olsen B A. J Chromatogr A,2001,913:113 |
| [63] | McCalley D V. J Chromatogr A,2008,1193:85 |
| [64] | Fountain K J,Xu J,Diehl D M,et al. J Sep Sci,2010,33:740 |
| [65] | Damme T V,Lachová M,Lynen F,et al. Anal Bioanal Chem,2014,406:401 |
| [66] | dos Santos Pereira A,Girón A J,Admasu E,et al. J Sep Sci,2010,33:834 |
| [67] | Gaber Y,Trnvall U,Kumar M A,et al. Green Chem,2011,13:2021 |
| [68] | dos Santos Pereira A,David F,Vanhoenacker G,et al. J Sep Sci,2009,32:2001 |
| [69] | Hayama T,Yoshida H,Todoroki K,et al. Rapid Commun Mass Spectrom,2008,22:2203 |
| [70] | Gritti F,dos Santos Pereira A,Sandra P,et al. J Chroma-togr A,2009,1216:8496 |
| [71] | Gritti F,dos Santos Pereira A,Sandra P,et al. J Chroma-togr A,2010,1217:683 |
| [72] | Dong L L,Huang J X. Chromatographia,2007,65:519 |
| [73] | Bicker W,Wu J Y,Lmmerhofer M,et al. J Sep Sci,2008, 31:2971 |
| [74] | Hao Z,Xiao B,Weng N. J Sep Sci,2008,31:1449 |
| [75] | Shou W Z,Naidong W. J Chromatogr B,2005,825:186 |
| [76] | Liu M,Chen E X,Ji R,et al. J Chromatogr A,2008,1188:255 |
| [77] | Li R P,Yuan Q,Zhang Y,et al. J Liq Chromatogr Rel Technol,2011,34:511 |
| [78] | Guo Y,Gaiki S. J Chromatogr A,2005,1074:71 |
| [79] | Chirita R-I,West C,Finaru A-L,et al. J Chromatogr A, 2010,1217:3091 |
| [80] | Wang P G,He W. Hydrophilic Interaction Chromatography (HILIC) and Advanced Applications. New York:CRC Press,2011 |
| [81] | Guo Y,Srinivasan S,Gaiki S. Chromatographia,2007,66:223 |
| [82] | Lundanes E,Greibrokk T. Adv Chromatogr,2006,44:45 |
| [83] | Zhu P L,Dolan J W,Snyder L R,et al. J Chromatogr A, 1996,756:51 |
| [84] | Louw S,Lynen F,Hanna-Brown M,et al. J Chromatogr A, 2010,1217:514 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%