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为建立中药材中氨基类极性非紫外活性成分的定性与定量分析方法，以中药材天南星为研究对象，采用柱前衍生化技术，以异硫氰酸苯酯（PITC）为衍生化试剂，经 C18色谱柱（100 mm×2.1 mm，3.5μm）分离和超高效液相色谱-串联质谱（ UHPLC-MS／MS）分析，共解析了天南星中20个成分，包括18个氨基酸和2个胺类化合物。经优化衍生化条件，应用高效液相色谱法（HPLC），以 Diamonsil C18色谱柱（250 mm×4.6 mm，5μm）分离，以乙腈和0.05 mol／L醋酸铵-醋酸缓冲液（ pH 6.5）为流动相，梯度洗脱，在254 nm 下检测，建立了同时测定15种氨基酸含量的方法，经方法学考察符合含量测定要求。谷氨酸、色氨酸在2~100 mg／L 范围内、精氨酸在6~300 mg／L 范围内、其余各氨基酸在0.8~40 mg／L范围内均呈良好的线性关系，相关系数均不小于0.9995；平均回收率在95％~105％之间，RSD均小于3％；并成功应用于12批中药材的测定。本方法简便、灵敏、准确，具有可操作性，可用于快速鉴定中药中的氨基类成分以及进行含量测定。

A method for the identification and determination of the polar amino components without ultraviolet activity in traditional Chinese medicines was developed. With Rhizoma Ari-saematis as the object of this study,using pre-column derivatization with phenyl isothiocyanate(PITC)as the derivatization reagent,compounds were separated and identified on a C 18 column (100 mm × 2. 1 mm,3. 5 μm)by ultra high performance liquid chromatography-tandem mass spectrometry( UHPLC-MS/MS ). A total of 20 components,including 18 amino acids and 2 amine compounds were identified. Furthermore,after the optimization of the derivatization conditions,15 amino acids were determined by high performance liquid chromatography(HPLC)on Diamonsil C18 column(250 mm×4. 6 mm,5 μm),detected at 254 nm and gradient-ly eluted by acetonitrile and 0. 05 mol/L ammonium acetate-acetic acid( pH 6. 5)as the mobile phases. The results of methodological study demonstrated that the method can meet the requirements of the determination. All calibration curves expressed good linearity:Glu,Try in the range of 2-100 mg/L,Arg in the range of 6-300 mg/L,others in the range of 0. 8-40 mg/L, with the correlation coefficients ≥ 0. 999 5. The average recovery of this method was among 95%-105% and the RSD was less than 3%. The developed method was successfully applied to quantitative determination of amino compounds in 12 batches of Rhizoma Arisaematis samples.The method is simple,sensitive,accurate,and can be used for rapid identification and deter-mination of amino components in traditional Chinese medicines.