建立了生物样品中8种毒品的超声辅助分散液液微萃取-气相色谱-三重四极杆串联质谱检测方法，采用密度比水低的有机溶剂甲苯作为萃取溶剂，萃取过程中不需要任何分散剂。对影响萃取富集效率的因素进行优化：将100μL甲苯萃取剂加入到1 mL样品溶液中，超声波剧烈振荡使甲苯充分分散到样品溶液中进行萃取，离心分层后，抽取上层萃取剂供气相色谱-三重四极杆串联质谱分析检测。在优化条件下，分析物在各自的线性范围内具有良好的线性关系，线性相关系数在0.9984~0.9994之间；检出限为0.05~0.40μg／L（ S／N=3）；样品加标回收率在79.3％~100.3％之间，RSD<5.7％。本方法具有操作简单、灵敏度高和重现性好等优点，可应用于生物样品中多种毒品的分析检测。
A novel microextraction technique based on ultrasound-assisted low-density solvent dispersive liquid-liquid microextraction( UA-LDS-DLLME)has been developed for the determi-nation of multiple drugs of abuse in biological samples by gas chromatography-triple quadrupole mass spectrometry( GC-QQQ-MS). A total of 100 μL of toluene as extraction solvent was dropped into the sample solution. Then the mixture was sonicated drastically in an ultrasonic bath for 3 min with occasional manual shaking to form a cloudy suspension. After centrifugation at 10 000 r/min for 3 min,the upper layer of low-density extractant was withdrawn and injected into the GC-QQQ-MS for analysis. The parameters affecting extraction efficiency have been investigated and optimized. Under the optimum conditions,good linearities were observed for all analytes with the correlation coefficients ranging from 0. 998 4 to 0. 999 4. The recoveries of 79. 3%—100. 3% with RSDs<5. 7% were obtained. The LODs( S/N=3)were in the range from 0. 05 to 0. 40 μg/L. UA-LDS-DLLME technique has the advantages of less extraction time,suit-able for batches of sample pretreatment simultaneously,and higher extraction efficiency. It was successfully applied to the analysis of amphetamines in real human urine samples.
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