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采用固液相表面连续反应法，先将偶联剂γ-[（2，3）-环氧丙氧]丙基三甲氧基硅烷（KH-560）键合到球形硅胶表面，然后再将植物有效成分丁香酚与硅胶上的 KH-560活性基团反应，合成了丁香酚键合硅胶液相色谱固定相（ EGSP）。采用元素分析、热重分析和红外光谱对该固定相的结构进行了表征。以萘作为溶质探针，乙腈-水（35：65，v／v）为流动相，流速为0.8 mL／min，测得 EGSP柱的柱效。以一系列的中性、碱性和酸性化合物为溶质探针， C18柱和苯基柱作参比，对该固定相的色谱性能及保留机理进行了研究。结果表明，硅胶表面成功键合上了丁香酚配体，键合量为0.28 mmol／g，EGSP柱理论塔板数约为24707 N／m。该固定相不仅具有良好的反相色谱性能，同时由于配体结构中含有芳环、烯基和甲氧基，还能与溶质发生π-π电荷转移、偶极-偶极和氢键作用。与传统的反相 C18柱和苯基柱相比，EGSP在极性芳香族化合物的快速、简便分离中占优势。

A eugenol-bonded silica gel stationary phase( EGSP ) for high performance liquid chromatography( HPLC)has been synthesized by the solid-liquid successive reaction method. The preparation process included two steps:firstly,γ-glycidoxypropyltrimethoxy-silane( KH-560)was covalently attached to the surface of spherical silica gel. Then the bonded silica gel continued to react with eugenol ligand,which was a plant active component,and obtained EGSP. The structure of EGSP was characterized by elemental analysis,thermogravimetric anal-ysis and Fourier transform infrared spectroscopy. Using naphthalene as a probe,the column efficiency was tested under the mobile phase of acetonitrile-water(35:65,v/v)at a flow rate of 0. 8 mL/min. The chromatographic properties and the retention mechanism of EGSP were evaluated by using neutral,basic and acidic analytes as solute probes. Meanwhile,the compar-ative study with C18 column and phenyl column was also carried out under the same chromato-graphic conditions. The result showed that the eugenol ligand was successfully bonded to the surface of silica gel with a 0. 28 mmol/g of bonded amount,and the theoretical plate number of EGSP column was about 24 707 N/m. The EGSP appeared to be a kind of excellent reversed-phase stationary phase with suitable hydrophobicity and various synergistic sites. The eugenol ligand bonded on silica gel could first provide π-π interaction sites for different analytes because of its benzene ring and alkenyl. In addition,the methoxy groups of eugenol were re-sponsible for dipole-dipole and hydrogen-bonding interactions between the ligand and solutes in the effective separation process. Comparing with traditional C 18 column and phenyl column,EGSP has an advantage in the fast separation of polar compounds under simple experimental conditions.