建立了黄酒中葡萄糖、果糖、异麦芽糖、异麦芽三糖、麦芽糖、潘糖、麦芽三糖共7种糖类的高效阴离子交换色谱分离-积分脉冲安培检测的方法。以氢氧化钠和醋酸钠为淋洗液进行梯度洗脱，CarboPacTM 10阴离子交换柱进行分离。该方法可在26 min内实现黄酒中7种糖类的定量分析，在0.5~50 mg／L浓度范围内线性关系良好，样品定量限为0.1 g／L，加标回收率为76.5％~108.4％，精密度（ RSD）为3.02％~8.23％。将该法用于不同厂家不同批次的绍兴加饭酒中各种糖类含量的检测，基于所得的检测结果，利用中位数法构建了标准指纹图谱，并采用夹角余弦法评价了各个样品与标准指纹图谱之间的相似度。结果初步显示，不同厂家加饭酒之间的相似度差别较大，该法可作为区别不同厂家酒的依据之一。
An analytical method for the determination of glucose,fructose,isomaltose,isomal-totriose,maltose,panose and maltotriose was developed by high performance anion exchange chromatography coupled with integrated pulsed amperometric detection. The analysis was per-formed on a CarboPacTM10 column with gradient elution of NaOH-NaOAc as mobile phases. The seven sugars were well separated in less than 26 min and showed good linear correlation coeffi-cients between 0. 5 and 50 mg/L. The detection limits( LODs)were 0. 1 g/L. The recoveries ranged from 76. 5% to 108. 4% at different spiked levels. The relative standard deviations were between 3. 02% and 8. 23%. The established method was applied in determining the seven sugars in different batches of Shaoxing Jiafan rice wines from different manufacturers. The standard fingerprints were established by median values using the obtained results. Similarities of the fin-gerprints of the samples to the standard were calculated by using angle cosine. The results showed that the similarities of wines from different manufacturers were significantly different, so the method could be used for the identification of rice wines from different manufacturers.
参考文献
| [1] | 王家林;张颖;于秦峰.黄酒中生物活性成分的探讨[J].酿酒科技,2011(7):47-50. |
| [2] | 倪莉;吕旭聪;黄志清;蔡琪琪;梁爽;饶平凡.黄酒的生理功效及其生理活性物质研究进展[J].中国食品学报,2012(3):1-7. |
| [3] | 李家寿.黄酒色、香、味成分来源浅析[J].酿酒科技,2001(03):48-50. |
| [4] | GB/T 13662-2008.[S]. |
| [5] | 李博斌;刘兴泉;吴坚;余燕飞;诸葛庆;郑云峰.黄酒中糖和无机元素成分与黄酒口味品质的定量关系研究[J].中国酿造,2010(8):37-40. |
| [6] | 谢广发;戴军;赵光鳌;陈尚卫;帅桂兰.黄酒中的功能性低聚糖及其功能[J].中国酿造,2005(2):39-40. |
| [7] | Yu H.;Ding YS.;Mou SF..Direct and simultaneous determination of amino acids and sugars in rice wine by high-performance anion-exchange chromatography with integrated pulsed amperometric detection[J].Chromatographia,200311/12(11/12):721-728. |
| [8] | GB/T 22222-2008.[S]. |
| [9] | 丁洪流;李灿;金萍;袁丽红;姚永青;陈英;李培.高效液相色谱-蒸发光散射法测定食品中的单糖、双糖、低聚果糖和糖醇[J].色谱,2013(8):804-808. |
| [10] | 周洪斌;熊治渝;李平;李静;孙莉;赵云霞.离子色谱-质谱联用法检测食品中的糖醇[J].色谱,2013(11):1093-1101. |
| [11] | Giancarlo V;Irene D;Alessandro C.[J].Rapid Communications in Mass Spectrometry,2009(23):1607. |
| [12] | 耿丽娟;黄峻榕;冯峰;蒋萍萍;储晓刚;张峰;凌云.高效阴离子交换色谱-脉冲安培检测法测定乳粉中的低聚果糖[J].色谱,2014(12):1380-1384. |
| [13] | 徐颖;臧颖;姜婷;郑兆娟;欧阳嘉.高效阴离子交换色谱-脉冲安培检测法同时测定生物转化样品中的海藻糖、葡萄糖和麦芽糖[J].色谱,2014(12):1400-1403. |
| [14] | 张蓉;司晓喜;伊奥尔;张红;丁中涛;张凤梅;朱瑞芝.高效阴离子交换色谱分离-积分脉冲安培检测法同时测定烟草薄片中的糖和保润成分[J].分析试验室,2014(8):963-966. |
| [15] | 牟世芬;于泓;蔡亚岐.糖的高效阴离子交换色谱-脉冲安培检测法分析[J].色谱,2009(5):667-674. |
| [16] | Martin R;Marco G;Gunther B.[J].Journal of Chromatography B,2015988:106. |
| [17] | Xie J H;Shen M Y;Nie S P.[J].Carbohydrate Polymers,201398:976. |
| [18] | Xi L L;Wang F L;Zhu Z Y.[J].TALANTA,2014119:440. |
| [19] | Mercier, G.;Campargue, C..Partially hydrolyzed guar gum characterization and sensitive quantification in food matrices by high performance anion exchange chromatography with pulsed amperometric detection-Validation using accuracy profile[J].Journal of chromatography, A: Including electrophoresis and other separation methods,2012:180-187. |
| [20] | Daniel R;Svein H K;Judit K.[J].Carbohydrate Research,2012358:56. |
| [21] | Manuel U C;Gonzalo C G;Irene L R.[J].Journal of Mathematical Chemistry,200640(1):15. |
| [22] | 张军翔;冯长根;李华.蛇龙珠葡萄酒酒龄花青素高效液相色谱（HPLC）指纹图谱研究[J].中国农业科学,2006(7):1451-1456. |
| [23] | 周围;周小平;赵国宏;刘红卫;高黎红;陈立仁;刘向东;唐煜.名优白酒质量指纹专家鉴别系统[J].分析化学,2004(6):735-740. |
| [24] | 祝成;张宿义;赵金松.不同感官等级白酒基酒的相似度研究[J].中国酿造,2011(9):157-160. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%