维药是祖国医药学不可分割的组成部分。维药现代化，即利用现代技术研究维药的有效成分，是维药科学化、标准化、规范化、商品化和产业化的必经之路。本文建立了维药蜀葵花中有效成分芦丁、槲皮素和山柰酚的选择性提取方法，优化了高效液相色谱法（HPLC）同时测定这3种有效成分的分析条件。采用 HC-C18色谱柱（250 mm×4.6 mm，5μm）和甲醇-0.4％磷酸（50∶50，v／v）流动相，在柱温30℃和流速1.00 mL／min的条件下实现了3种物质之间以及和干扰物之间的基线分离。维药蜀葵花中芦丁、槲皮素及山柰酚的线性范围分别为12.5~150μg／mL（ r＝0.9998），12.5~125μg／mL（ r＝0.9999）及12.5~125μg／mL（ r＝0.9988），加标回收率（ n＝5）分别为100.3％（ RSD＝1.1％）、97.60％（ RSD＝0.47％）、97.75％（ RSD＝0.71％）。该方法实现了同时测定维药蜀葵花中芦丁、槲皮素及山柰酚，为其他黄酮类物质的开发应用提供了科学依据，同时也可为其他维药分析提供借鉴。
Uyghur medicine is one important part of the national medicine system. Uyghur med-icine modernization,namely the study of effective components with modern technologies,is the only way for the scientification,standardization,and industrialization of Uyghur medicine. Here we developed a selective extraction method for rutin,quercetin and kaempferol in Althaea rosea( L)Gavan. The three active species were determined by high performance liquid chroma-tography(HPLC)with HC-C18 column(250 mm×4. 6 mm,5 μm)and the mobile phase of CH3OH-0. 4% H3PO4(50∶50,v/v). Rutin,quercetin and kaempferol were baseline separated with each other and the interference species with flow rate of 1. 0 mL/min and column tempera-ture of 30 ℃. Under the optimal conditions,linear correlation were obtained in the mass con-centration range of 12. 5-150 μg/mL( r=0. 999 8)for rutin,12. 5-125 μg/mL( r=0. 999 9)for quercetin,and 12. 5-125 μg/mL( r=0. 998 8)for kaempferol. The recoveries( n=5)of rutin, quercetin and kaempferol were 100. 25%( RSD=1. 1%),97. 60%( RSD=0. 47%)and 97. 75%( RSD=0. 71%),respectively. The method can be used to determine the contents of rutin,quer-cetin and kaempferol in Althaea rosea( L)Gavan and provide the guidance for the analysis of the flavonoids in other Uyghur medicines.
参考文献
| [1] | Parvu M;Toiu A;Vlase L;Alina Parvu E.Determination of some polyphenolic compounds from Allium species by HPLC-UV-MS.[J].Natural product research,201014(14):1318-1324. |
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