采用超临界流体色谱法（ SFC），在多糖固定相 Chiralpak IA、IB、IC、ID、IE和IF上成功拆分了11种手性化合物。分离结果表明，这6支手性色谱柱对这些手性化合物具有良好的手性识别互补性，均可以在10 min 之内得到良好的分离结果，具有较好的实用性。改性剂甲醇、乙醇和异丙醇对手性化合物的保留时间以及手性选择性均具有良好的调节作用，需要根据不同手性物质在手性柱上的分离情况加以区别，选择使用，并调节改性剂至合适的比例。针对键合型固定相溶剂通用性的特征，特殊改性剂的应用也有助于优化手性分离。
Eleven kinds of chiral compounds have been well separated within 10 min on poly-saccharide derivative-based chiral stationary phases named Chiralpak IA,IB,IC,ID,IE and IF by supercritical fluid chromatography( SFC). The chiral recognition of these chiral compounds has demonstrated good complementary enantioselectivities of the six chiral columns,which were proved to be useful for chiral SFC. Both the elution time and enantioselectivies could be significantly affected by the modifier types and their concentrations,such as methanol,ethanol and isopropanol,which should be optimized during the experiments. In addition,the solvent versatility of the immobilized chiral stationary phase on the optimization of the chiral separation was helpful.
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