近年来随着生命科学等领域的深入发展，人们对微量样本分离分析的需求越来越高，液相色谱系统的微量化受到了更多的关注。由于开管毛细管色谱柱具有较低的反向压力，可通过采用更长的色谱柱提高柱效，从而实现对复杂生物样本的高效分离，因而成为液相色谱柱新的发展方向。本文对开管毛细管色谱柱的制备方法及应用进展进行了综述。
With the further development of life sciences in recent years,the demand for analy-sis of very small amounts of sample has increased,and more attention has been paid for the miniaturized liquid chromatography system. Longer open tubular capillary columns were selected to increase column efficiency due to their low column back pressure,and they were often used to separate complex biological samples in high resolution. Therefore,they emerged as the new area of liquid chromatography columns. The preparation methods and applications in proteomics of open tubular columns in capillary liquid chromatography( capLC)are reviewed.
参考文献
| [1] | Tabata, K.;Jinno, N.;Noda, K.;Hashimoto, M.;Tsukagoshi, K..Tentative comparison of tube radial distribution chromatography and CZE[J].Chromatographia,20127/8(7/8):423-428. |
| [2] | Guo Y.;Colon LA..A STATIONARY PHASE FOR OPEN TUBULAR LIQUID CHROMATOGRAPHY AND ELECTROCHROMATOGRAPHY USING SOL-GEL TECHNOLOGY[J].Analytical chemistry,199515(15):2511-2516. |
| [3] | Nischang, I.;Brueggemann, O.;Svec, F..Advances in the preparation of porous polymer monoliths in capillaries and microfluidic chips with focus on morphological aspects[J].Analytical and bioanalytical chemistry,20103(3):953-960. |
| [4] | Luo Q;Gu Y;Wu SL;Rejtar T;Karger BL.Two-dimensional strong cation exchange/porous layer open tubular/mass spectrometry for ultratrace proteomic analysis using a 10 mum id poly(styrene- divinylbenzene) porous layer open tubular column with an on-line triphasic trapping column.[J].Electrophoresis: The Official Journal of the International Electrophoresis Society,20088(8):1604-1611. |
| [5] | Thakur, D.;Rejtar, T.;Wang, D.;Bones, J.;Cha, S.;Clodfelder-Miller, B.;Richardson, E.;Binns, S.;Dahiya, S.;Sgroi, D.;Karger, B.L..Microproteomic analysis of 10,000 laser captured microdissected breast tumor cells using short-range sodium dodecyl sulfate-polyacrylamide gel electrophoresis and porous layer open tubular liquid chromatography tandem mass spectrometry[J].Journal of chromatography, A: Including electrophoresis and other separation methods,201145(45):8168-8174. |
| [6] | Collins, D.A.;Nesterenko, E.P.;Brabazon, D.;Paull, B..Fabrication of bonded monolithic porous layer open tubular (monoPLOT) columns in wide bore capillary by laminar flow thermal initiation[J].Chromatographia,201311/12(11/12):581-589. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%