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建立了超高效液相色谱-四极杆-飞行时间串联质谱（ UPLC-Q-TOF／MS）快速筛查血液中10种毒物的检测方法。用乙酸乙酯提取血液样品，浓缩至近干后，用甲醇溶解定容，过0.22μm 滤膜后，直接测定。目标物经 ACQU-ITY UPLC@BEH C18色谱柱（100 mm×2.1 mm，1.7μm）分离，以甲醇和0.1％（v／v）甲酸水溶液为流动相，梯度洗脱，四极杆飞行时间串联质谱电喷雾正离子模式检测，外标法定量。结果表明：目标物在10.0~500.0μg／L 范围内线性关系良好，相关系数为0.9908~0.9958，检出限和定量限分别为1.0~2.0μg／L 和4.0~8.0μg／L。在20.0、50.0和200.0μg／L 3个添加水平下的平均回收率为56.7％~83.0％，相对标准偏差为3.6％~8.9％。利用Agi-lent Mass Hunter PCDL Manager软件建立常见毒物的数据库，并应用于加标样品的筛查分析。该方法能快速筛查出添加的10种常见毒物，检出率达100％，且保留时间偏差均小于0.1 min，质量偏差均小于1 mDa，同位素丰度匹配得分、同位素间距得分和 MS匹配得分均大于90，MS／MS 图谱匹配得分均大于70。该方法可用于法庭与临床毒物分析。

A high-throughput method was developed for rapid screening of 10 drugs in blood by ultra performance liquid chromatography with high resolution quadrupole-time-of-flight mass spectrometry( UPLC-Q-TOF/MS). The sample was extracted by ethyl acetate,the extraction solution was concentrated to near dryness,and dissolved with methanol. Then the sample was passed through a 0. 22 μm membrane. The separation of the 10 target compounds was per-formed on a Waters ACQUITY UPLC@BEH C18 column(100 mm×2. 1 mm,1. 7 μm)with gra-dient elution using methanol and 0. 1%( v/v)formic acid aqueous solution as mobile phases, and analyzed by UPLC-Q-TOF/MS under electrospray ionization ( ESI ) mode with scanning range of m/z 50-1 000. Rapid screening can be achieved using MS matching scores,deviation of retention time,measured mass,isotopic abundance matching scores,isotope spacing match scores and MS/MS matching scores. Good linearities were observed in the range of 10. 0-500. 0μg/L with the correlation coefficients from 0. 990 8 to 0. 995 8. The limits of detection and the limits of quantification were 1. 0-2. 0 μg/L and 4. 0-8. 0 μg/L,respectively. The spiked recov-eries were 56. 7% -83. 0% with the relative standard deviations of 3. 6% -8. 9%. The result screening database was built using Agilent Mass Hunter PCDL Manager software and then used for the analysis of spiked samples. MS matching scores,isotopic abundance matching scores, isotope spacing matching scores(all > 90 points)and MS/MS matching scores(> 70 points) were applied to identify the drugs. The results showed that all the spiked drugs could be cor-rectly identified with low deviations of retention time(< 0. 1 min)and mass(< 1 mDa). The developed method is suitable for the screening and confirmation of the drugs in forensic and clinical analytical toxicology.