糖蛋白及与蛋白质结合的糖链在生物体内的丰度一般极低，为实现其准确定性及定量，去除高丰度非糖蛋白、非糖肽的干扰，通常需要进行富集前处理操作。该文设计并制备了一种基于点击化学反应和烯丙基氯多功能化位点聚合物的葡萄糖改性核-壳-壳型磁性纳米颗粒，并建立了相应的用于糖肽亲水富集的固相微萃取方法。以人免疫球蛋白G酶解液为样品，共测得32个糖肽信号。该方法检出限可达10 fmol，每克磁性分离介质的富集容量达100 mg。
The abundance of glycoproteins and the glycans attached are usually low in body. In order to analyze them accurately and eliminate the interference of non-glycoproteins and non-glycopeptides, methods of enrichment are needed. A kind of novel magnetic nanoparticles based on click reaction and multiple-functional-site poly-(allyl chloride) structure is designed and prepared. The related hydrophilic enrichment method for glycopeptide is developed. Totally 32 glycopeptide signals were detected from human immunoglobulin G trypsin enzymolysis under the optimized conditions. The method can reach a limit of detection of 10 fmol on human immunoglobulin G trypsin enzymolysis, with an adsorption capacity of 100 mg of 1 g particles.
参考文献
| [1] | Feng S, Yang N, Pennathur S, et al.. Anal Chem, 2009, 81(10): 3776 |
| [2] | Liu Y, Ren L, Liu Z.. Chem Commun, 2011, 47(17): 5067 |
| [3] | Liu Y, Lu Y, Liu Z.. Chem Sci, 2012, 3(5): 1467 |
| [4] | Li L, Lu Y, Bie Z, et al.. Angew Chem Int Ed, 2013, 52(29): 7451 |
| [5] | Zhang H, Li X J, Martin D B, et al.. Nat Biotechnol, 2003, 21(6): 660 |
| [6] | Zhu J, Wang F, Cheng K, et al.. Proteomics, 2013, 13(8): 1306 |
| [7] | Kolarich D, Jensen P H, Altmann F, et al.. Nat Protoc, 2012, 7(7): 1285 |
| [8] | Li Y, Zhang X, Deng C.. Chem Soc Rev, 2013, 42(21): 8517 |
| [9] | Pan Y, Du X W, Zhao F, et al.. Chem Soc Rev, 2012, 41(7): 2912 |
| [10] | Guo J, Yang W, Wang C.. Adv Mater, 2013, 25(37): 5196 |
| [11] | Xiong Z C, Qin H Q, Wan H, et al.. Chem Commun, 2013, 49(81): 9284 |
| [12] | Chen Y J, Xiong Z C, Zhang L Y, et al.. Nanoscale, 2015, 7(7): 3100 |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%