血清中磷酸化肽种类和浓度的变化既能反映人体内蛋白质水解酶活性的变化,又能反映蛋白质翻译后磷酸化的水平,业已成为肿瘤标志物寻找和发现的重要目标.因而,血清中磷酸化肽的鉴定及其定量分析在具有临床应用价值的肿瘤标志物的筛选与发现中起着重要作用.由于血清中的内源性磷酸化肽丰度极低,在质谱分析中的离子化效率不高,且受到来自高丰度非磷酸化肽和蛋白质的信号抑制及干扰,血清中磷酸化肽的质谱定量分析是分析化学研究中的一个巨大挑战.文章对血清磷酸化肽的分离富集、质谱定量分析及其作为肿瘤标志物的筛选和评价等3个方面的研究进展进行总结、评述,并展望该领域的未来研究趋势和应用前景.
参考文献
| [1] | Paradela A;Albar JP.Advances in the analysis of protein phosphorylation[J].Journal of proteome research,20085(5):1809-1818. |
| [2] | Feng S;Ye M;Zhou H;Jiang X;Jiang X;Zou H;Gong B.Immobilized zirconium ion affinity chromatography for specific enrichment of phosphopeptides in phosphoproteome analysis.[J].Molecular & cellular proteomics: MCP,20079(9):1656-1665. |
| [3] | Wu, J.-H.;Li, X.-S.;Zhao, Y.;Gao, Q.;Guo, L.;Feng, Y.-Q..Titania coated magnetic mesoporous hollow silica microspheres: Fabrication and application to selective enrichment of phosphopeptides[J].Chemical communications,201047(47):9031-9033. |
| [4] | Gygi,SP;Rist,B;Gerber,SA;Turecek,F;Gelb,MH;Aebersold,R.Quantitative analysis of complex protein mixtures using isotope-coded affinity tags.[J].Nature biotechnology,199910(10):994-999. |
| [5] | Thompson A.;Schafer J.;Kuhn K.;Kienle S.;Schwarz J.;Schmidt G. Neumann T.;Hamon C..Tandem mass tags: A novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS[J].Analytical Chemistry,20038(8):1895-1904. |
| [6] | Wu, C.-J.;Chen, Y.-W.;Tai, J.-H.;Chen, S.-H..Quantitative phosphoproteomics studies using stable isotope dimethyl labeling coupled with IMAC-HILIC-nanoLC-MS/MS for estrogen-induced transcriptional regulation[J].Journal of proteome research,20113(3):1088-1097. |
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