蛋白质糖基化是一种广泛存在的重要的蛋白质翻译后修饰,糖基化肽在总酶解肽中占的比例不超过5％,这使得糖肽的分离富集成为糖蛋白质组学研究发展的关键技术之一.在诸多糖蛋白质组富集技术中,化学方法是富集技术的主导,本文从化学反应的角度介绍糖基化蛋白质富集的技术进展.富集过程按照连接和释放分别讨论,在连接过程中,重点介绍硼酸化学法、肼化学法、胺化学法和肟点击法;在释放过程中,以N-糖蛋白的酶释放法和O-糖蛋白的β-消除法为主导,一并介绍了最新的氧化断裂释放的化学法.最后,讨论总体富集策略的发展现状.该文以糖蛋白富集的共价反应为核心,分析不同方法的优缺点以及各技术在糖蛋白质组学研究中的应用和贡献.
参考文献
| [1] | Drake RR;Schwegler EE;Malik G;Diaz J;Block T;Mehta A;Semmes OJ.Lectin capture strategies combined with mass spectrometry for the discovery of serum glycoprotein biomarkers.[J].Molecular & cellular proteomics: MCP,200610(10):1957-1967. |
| [2] | Ball LE;Berkaw MN;Buse MG.Identification of the major site of O-linked beta-N-acetylglucosamine modification in the C terminus of insulin receptor substrate-1.[J].Molecular & cellular proteomics: MCP,20062(2):313-323. |
| [3] | Cao, J;Shen, CP;Wang, H;Shen, HL;Chen, YC;Nie, AY;Yan, GQ;Lu, HJ;Liu, YK;Yang, PY.Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach[J].Journal of proteome research,20092(2):662-672. |
| [4] | Tran, T.H.;Park, S.;Kim, B.;Kim, O.-H.;Oh, B.-C.;Lee, D.;Lee, H..Ultrasmall gold nanoparticles for highly specific isolation/enrichment of N-linked glycosylated peptides[J].The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry,20124(4):991-998. |
| [5] | Abdelmagid AF.;Harris BD.;Maryanoff CA.;Shah RD.;Carson KG..REDUCTIVE AMINATION OF ALDEHYDES AND KETONES WITH SODIUM TRIACETOXYBOROHYDRIDE - STUDIES ON DIRECT AND INDIRECT REDUCTIVE AMINATION PROCEDURES[J].The Journal of Organic Chemistry,199611(11):3849-3862. |
| [6] | Harazono A;Kawasaki N;Itoh S;Hashii N;Ishii-Watabe A;Kawanishi T;Hayakawa T.Site-specific N-glycosylation analysis of human plasma ceruloplasmin using liquid chromatography with electrospray ionization tandem mass spectrometry[J].Analytical Biochemistry: An International Journal of Analytical and Preparative Methods,20062(2):259-268. |
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