建立了微乳液相色谱(MELC)同时测定中成药及保健品中添加的10种降压药物的分析方法.考察了微乳流动相的组成成分(包括表面活性剂、助表面活性剂、油相的种类及质量分数)、pH值和固定相种类对分离效果的影响.同时利用星点设计-响应面优化法进一步优化微乳组成.实验得到的最佳分离条件:色谱柱为Agela Promosil C18(2)(250 mm×4.6 mm,5μm);微乳流动相为5.6％(质量分数)十二烷基聚乙二醇醚(Brij35)-0.17％(质量分数)十二烷基硫酸钠(SDS)-5.1％(质量分数)正丁醇-0.46％(质量分数)环己烷-水(pH 3.0);流速为1.0mL/min;检测波长为230 nm;柱温为30℃.该方法具有灵敏度高、稳定性好和环保的优点.
A method was developed for simultaneous determination of 10 spiked chemical drugs in antihypertensive herb preparations and health foods by microemulsion liquid chromatography (MELC).The nature and mass fraction of microemulsion components (surfactant,co-surfactant,and oil phase),the pH of microemulsion,and the type of the stationary-phase were investigated.Central composite design-response surface methodology was used.The optimized conditions for the separation of the 10 spiked chemical drugs were as follows:an Agela Promosil C18(2) column (250 mm×4.6 mm,5 μm) was used at 30 ℃,and the microemulsion mobile phase (pH 3.0) consisted of 5.6％ (mass fraction) polyoxyethylene lauryl ether (Brij35),0.17％ (mass fraction) sodium dodecylsulfate (SDS),5.1％ (mass fraction) n-butanol,0.46％ (mass fraction) cyclohexane and water (pH 3.0).The method is efficient,sensitive and environment friendly.
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