建立了超高效液相色谱-飞行时间-串联质谱(UPLC-TOF-MS/MS)分析大白菜叶片中脂质的种类、结构、脂肪酸组成并检测其相对含量的方法.以Acquity UPLCTM BEH C8色谱柱为固定相,采用飞行时间质谱全扫描-信息关联采集-子离子扫描(TOF-MS scan-IDA-product ion scan)复合模式实现一次进样分析,同时获得脂质的一级和二级质谱信息.在大白菜叶片中共鉴定得到232种脂质,包含104种磷脂、63种糖脂和65种甘油酯.其中,磷脂、糖脂和甘油酯中的主要成分分别为磷脂酰胆碱、单半乳糖二酰甘油和甘油二酯、甘油三酯.结果表明,该方法具有灵敏度高、准确度高和高通量等优点,为植物脂质的代谢研究提供了可靠的分析技术平台,并为进一步的脂质生物学功能研究奠定了基础.
A method for the lipid analysis of Chinese cabbage leaves was developed by ultra performance liquid chromatography-time of flight-tandem mass spectrometry (UPLC-TOF-MS/MS).The lipid species,structures,fatty acid compositions and relative contents in Chinese cabbage leaves were elucidated.Acquity UPLCTM BEH C8 column was selected as the stationary phase.A fast and efficient chromatographic separation was accomplished within 22 min.TOF-MS scaninformation dependent acquisition-product ion scan was performed in ESI positive and negative ion modes to acquire high resolution MS and MS/MS spectra in one injection.Finally,232 lipids in Chinese cabbage leaves were identified,including 104 phospholipids,63 glycolipids and 65 glycerides.Among them,phosphatidylcholines and monogalactosyldiacylglycerols were chiefly phospholipids and glycolipids,respectively.Meanwhile,glycerides mainly contained diacylglycerols and triacylglycerols.The results demonstrated that the method for lipid analysis has high sensitivity,good accuracy and high throughput.It provides a reliable analysis platform for lipid metabolism in plants and lay a foundation for further study on biological functions of lipids.
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