制备了一种新型荧光分子印迹膜(L-半胱氨酸修饰的量子点嵌入的分子印迹膜(QDs@MIM)),并将其作为荧光人工受体用于目标蛋白质(溶菌酶)的特异性识别和检测.QDs@MIM以溶菌酶为模板分子、丙烯酰胺为功能单体、L-半胱氨酸修饰的量子点为辅助单体、N,N′-亚甲基双丙烯酰胺为交联剂,在预硅烷化的玻璃板上制备而成.在最佳条件下,QDs@MIM对溶菌酶检测的线性范围为0.1~1.0 μmol/L,吸附平衡时间为4 min,选择性因子为6.2.该方法操作简单、吸附平衡时间短、选择性高,具备作为生物传感器快速分析样品中目标蛋白质的潜力.
A new method for producing L-cysteine-capped ZnS quantum dots (QDs) embedded molecularly imprinted membrane (QDs@MIM) was developed.The QDs@MIM was used as fluorescence artificial receptor for specific recognition and detection of target proteins.The QD@MIM was fabricated on a silylanized glass plate.Lysozyme, acrylamide, L-cysteine modified Mn2+-doped ZnS QDs and N,N′-methylenediacrylamide were used as template, functional monomer, assistant monomer and cross-linker, respectively.Under optimal conditions, the linear range for lysozyme ranged from 0.1 to 1.0 μmol/L, adsorption time was 4 min and the imprinting factor for lysozyme was 6.2.The results showed that the molecularly imprinted polymer can be used as a simple, rapid and selective biosensor to detect target proteins in biologic samples.
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