建立了气相色谱-三重四极杆质谱(GC-MS/MS)同时测定烟丝中73种香气物质的分析方法.样品用无水乙醚振荡提取,提取液经过滤后直接进入DB-5MS色谱柱(30 m×0.25 mm, 0.25 μm)分离,通过优化质谱参数,可有效降低复杂基质和重叠峰的干扰,同时采用多反应监测(MRM)模式测定,内标法定量.结果表明,目标物在各自的线性范围内线性关系良好(r2>0.99),低、中、高3个水平的加标回收率为70.0%~122.3%,相对标准偏差(RSD)为1.6%~20.4%.该方法具有前处理简单、准确灵敏的特点,适用于烟丝样品中73种香气物质的检测.
A method for simultaneous determination of 73 flavor components in cut tobacco by gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed.The samples were extracted with anhydrous ether, and the ether extracts were separated on a DB-5MS column (30 m×0.25 mm, 0.25 μm).The interference of complex matrix and peak overlapping of various flavor components were reduced by means of optimizing tandem mass spectrometry parameters.The extracts were analyzed by GC-MS/MS under multiple reaction monitoring (MRM) mode, and internal standard method was applied to quantify the 73 flavor components.The results showed that all the flavor components had good linearity in certain ranges with the correlation coefficients (r2) greater than 0.99.The recoveries of the 73 flavor components at low, middle and high levels ranged from 70.0% to 122.3% with the relative standard deviations (RSDs) of 1.6%-20.4%.The developed method is easy, accurate and sensitive, and it can be applied to the determination of the 73 flavor components in cut tobacco.
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