目的采用联合磁性微球和基质辅助的激光解吸/离子化-飞行时间（MALDI-TOF-MS）技术筛选肾细胞癌患者血清差异蛋白峰。方法"采用弱阳离子磁性微球从99份血清标本（肾细胞癌62份，肾脏良性占位病变37份）捕获血清蛋白，MALDI-TOF-MS质谱仪检测蛋白峰，Biomarker Wizard 3.1和Biomarker Patterns Software 5.0分析数据，以47例肾细胞癌及26例肾脏良性占位病变标本建立肾细胞癌诊断模型，15例肾细胞癌和11例肾脏良性占位病变标本进行盲法验证。结果"发现7个蛋白峰具有统计学差异（P<0.05），以质荷比2945.35 、15340.8、6984.51、5819.23的4个蛋白峰建立肾细胞癌诊断模型，该模型区分肾细胞癌与肾脏良性占位病变的敏感性为83.0%，特异性为84.6%；盲法验证，该模型诊断肾细胞癌的敏感性为80.0%，特异性为81.8%。 结论"采用磁性微球与MALDI-TOF-MS技术可以检测肾细胞癌血清中的差异蛋白峰，并建立敏感性、特异性较高的肾细胞癌诊断模型。
Objective To screen for the differential protein peaks of renal cell carcinoma (RCC) using magnetic beads-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MethodsSerum proteins were profiled by magnetic beads (WCX) from 62 RCC patients and 37 patients with benign renal space-occupying lesions. Protein peaks were identified by MALDI-TOF-MS. Data were analyzed with Biomarker Wizard 3.1 and Biomarker Patterns Software 5.0. Diagnostic model for RCC was constructed based on 47 RCC cases and 26 patients with benign renal space-occupying lesions. The remaining 26 cases were evaluated with blind method. ResultsSeven differential protein peaks related to RCC were identified (P<0.05). The diagnostic model for RCC constructed by the differential protein peaks (m/z〖HT〗〖HJ〗〖HK〗〖CD10〗〖HT6，7.5〗基金项目：国家自然科学基金（30772165）和高等学校博士学科点专项科研基金（20070023069）Supported by the National Natural Sciences Foundation of China（30772165）and the Research Fund for the Doctoral Program of Higher Education of China（20070023069）〖HT〗〖HJ〗〖GK2!2〗2945.35, 15340.8, 6984.51, and 5819.23) generated excellent separation between the RCC and control groups, with a sensitivity of 83.0% and the specificity of 84.6%. As validated by blind method, the model had a sensitivity of 80.0 % and a specificity of 81.8%. ConclusionDifferential protein peaks for RCC can be identified in serum by magnetic beads-based MALDI-TOF-MS, which is also valuable for the establishment of a RCC diagnostic model with a high sensitivity and specificity."
参考文献
| [1] |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%