合成了新型三脚架结构配体E(2-苯甲酰胺乙基)胺及其稀土Eu(3+)配合物.通过元素分析、IR、1H-NMR、TG-DTA和摩尔电导对此化合物进行组成和结构推测,结果显示配合物[EuL(NO3)2]·NO3是1:1型配位,3个硝酸根中有两个处于配位内界与Eu(3+)配位,总配位数为8.通过紫外吸收光谱、荧光发射光谱、粘度法及其与溴化乙啶(EB)的竞争实验研究了配合物与ct-DNA的作用情况,结果显示,配合物与ct-DNA作用时,其紫外吸收产生明显增色效应,荧光强度增强;EB-DNA体系的荧光强度随配合物的加入迅速减弱;配合物的加入使ct-DNA的粘度增加;实验结果证明,配合物与ct-DNA以插入方式结合,其键合常数Kq=8.86×10(3)L·mol(-1).凝胶电泳实验表明配合物[EuL(NO3)2]·NO3不能将pUC19 DNA选择性的断裂成线型和开环型DNA,并分析讨论了其原因.
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