在pH值为5.0～8.2的水溶液介质中,阳离子花青染料喹哪啶蓝(QB)在核酸分子表面形成了H-聚集. 随着核酸小牛胸腺DNA(ctDNA)或酵母RNA(yRNA)的加入,QB在598 nm处的吸收峰发生明显的减色效应. 据此以QB为探针,对核酸进行了定量测定. 测试条件为:QB 7.0 μmol/L;λ=598 nm;pH=7.3. 结果表明,ctDNA和yRNA的线性响应范围分别为8.0×10-8～2.0×10-6 g/mL和1.0×10-7～2.0×10-6 g/mL. 其5次平行测定的相对标准偏差分别为3.4%和3.6%.
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