采用硫酸铵分级沉淀和柱层析方法,从黑麦草叶片中分离得到纯的铜锌超氧化物岐化酶. 经鉴定该酶是Cu*Zn-SOD. 测得其分子量约为32 100,亚基分子量约15 900. 该酶的N-末端为丙氨酸,其紫外光区最大吸收峰在254 nm. 该酶的热稳定性较好,65 ℃保温1 h仍保留活性27%. 氨基酸组成分析表明,黑麦草SOD由212个氨基酸残基组成,而且不含色氨酸.
参考文献
| [1] | YANG Rong-Ying(杨荣英),ZHAO Xian-Hua(赵先华),ZHU You-Lin(朱友林). J Nanchang Univ(Natural Science)(南昌大学学报(理科版))[J],1996,20(4):324 |
| [2] | WANG Zhuan-Hua(王转花),ZHANG Zheng(张政),LIN Ru-Fa(林汝法), et al. J Chin Biochem(生物化学杂志)[J],1994,10(5):539 |
| [3] | ZOU Guo-Lin(邹国林),LUO Shi-Wen(罗时文),RONG Jun(荣俊), et al. Acta Biochem ET Biophysica Sinica(生物化学与生物物理学报)[J],1992,24(2):51 |
| [4] | XIE Wei-Hua(谢卫华),YAO Ju-Fang(姚菊芳),YUAN Qin-Sheng(袁勤生). J Chin Medicine Ind(中国医药工业杂志)[J],1988,19(5):217 |
| [5] | Beauchamp C,Fridovich I. Anal Biocchem[J],1971,44:276 |
| [6] | PENG Xiu-Ling(彭秀玲),YUAN Han-Ying(袁汉英),XIE Yi(谢毅), et al. Genetic Engineering Experiment Technique(基因工程实验技术)[M]. Changsha(长沙):Hunan Science and Technology Press(湖南科学技术出版社),1997:264 |
| [7] | ZHAO Yong-Fang(赵永芳). Application of the Biochemistry Technical Principia(生物化学技术原理及其应用)[M]. Wuhan(武汉): Wuhan University Press(武汉大学出版社),1988.343:65 |
| [8] | LIN Yuan-Zao(林元藻),WANG Feng-Shan(王凤山),WANG Zhuan-Hua(王转花), Chief-Edrs(主编). Biochemical Pharmaceutics(生化制药学)[M]. Beijing (北京):People Hygiene Press(人民卫生出版社),1998:207 |
| [9] | Liu Y,Zhang P B. Acta Zoological Sinica[J],1999,45(1):64 |
| [10] | ZOU Guo-Lin(邹国林),ZHU Ru-Pan(朱汝藩), Chief-Edrs(主编). Enzymology(酶学)[M]. Wuhan(武汉):Wuhan University Press(武汉大学出版社),1997:47 |
| [11] | Shao Chengbin. Fifth International Symposium on Green Chemistry[C],Hefei,China,2002:136 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%