应用高效液相色谱法/二极管阵列检测器同时测定了飞机草中木犀草素、槲皮素和山柰酚的含量.色谱柱HiQ sil C18W柱(4.6 mm×25 cm,5μm),流动相V(甲醇)∶V(水)∶V(磷酸)=50∶49.8∶0.2,检测波长槲皮素254 nm,木犀草素和山柰酚360 nm,温度30℃,流速1 mL/min,进样量10μL.确定了以超声波提取法制备飞机草分析样品的方法:溶剂为体积分数85%的乙醇,液固比为10∶1(mL/g),提取时间为1 h.结果表明,3种黄酮在0.01×10-3～0.10×10-3 g/mL范围内呈现良好线性关系(R2＞0.999 0),平均加样回收率分别为99.601 7%、99.032 6%和99.450 8%,RSD＜2%.该方法操作简便、准确度高,可快速测定飞机草中木犀草素、槲皮素和山柰酚3种物质的含量.
参考文献
| [1] | CHEN Ze-Tan(陈泽坦).Agrochemicals(农药)[J],2003,42 (4):45 |
| [2] | DING Zhi-Hui (丁智慧),ZHANG Xue-Mei (张学镅),LIU Ji-Kai (刘吉开),DING Jing-Kai (丁靖凯),GU Kun (古昆).Nat Prod Res Dev(天然产物研究与开发)[J],2001,13(5):22 |
| [3] | YUAN Jing-Quan(袁经权),YANG Jun-Shan(杨俊山),MIAO Jian-Hua(缪剑华).Chinese Traditi Herb Drugs (中草药)[J],2005,36(12):1 771 |
| [4] | HOU Chun-Lian(侯春莲),FU Yu-Jie(付玉杰),ZU Yuan-Gang(祖元刚),YANG Feng-Jian(杨逢建),ZHAO Wen-Hao(赵文灏).Bull Bot Res(植物研究)[J],2006,26(3):374 |
| [5] | HU Bi-Bo(胡碧波),JIANG Hui-Di(蒋惠娣),YANG Jun(杨俊),ZENG Su(曾苏).J Zhejiang Univ(淅江大学学报)[J],2004,33(1):29 |
| [6] | WANG Yan-Fang(王艳芳),WANG Xin-Hua(王新华),ZHU Yu-Tong(朱宇同).Nat Prod Res Dev(天然产物研究与开发)[J],2003,15(2):171 |
| [7] | ZHAO Xiao-Hui(赵晓辉),JIANG Fu-Quan(蒋福全),ZHOU Jian-Bo(周建波),WEN Huai-Xiu(文怀秀),SHAO Yun (邵赟),TAO Yan-Duo(陶燕铎).Chinese J Anal Lab(分析试验室)[J],2007,26(1):46 |
| [8] | QIAO Chun-Feng(乔春峰),HAN Quan-Bin(韩全斌),SONG Jing-Zheng(宋景政),DAI Jin (戴晋),MO Shi-Fu(莫世甫),XU Hong-Xi (徐宏喜).Chinese Pharm J(中国药学杂志)[J],2006,41(17):1 342 |
| [9] | SONG Shuang-Hong (宋双红),ZHANG Yuan (张媛),WANG Zhe-Zhi (王喆之).China J Chinese Materia Medica (中国中药杂志)[J],2006,31 (7):598 |
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