在pH=6.2的Na2HPO4-NaH2PO4缓冲溶液中,羊抗人纤维蛋白原血清与纤维蛋白原(Fg)发生特异性结合,产生抗体抗原免疫复合物微粒,导致体系在340 nm处的共振散射峰增强. 当纤维蛋白原质量浓度在1.33×10-7～5.33×10-6 g/mL范围内,随着纤维蛋白原浓度c的增加340 nm处的共振散射强度线性增强,回归方程为ΔI340 nm=12.17c+3.35,相关系数为0.998 6,其检出限(3σ)为2.8×10-8 g/mL Fg. 考察了18种共存物质的干扰. 结果表明,方法具有较高的选择性. 方法用于定量分析人血浆中的纤维蛋白原,相对标准偏差在2.2%～2.3%之间,测定结果与人体正常血浆中纤维蛋白原含量基本一致.
参考文献
| [1] | XU Xiu-Cai(徐修才).Sect Clin Biochem & Lab Med Foreign Med Sci(国外医学临床生物化学与检验学分册)[J],2004,25(6):503 |
| [2] | Acevedo M,Foody J M,Pearce C L.Am Heart J[J],2002,143:277 |
| [3] | Drouet L.Cerebrovascular Diseases Foreign Med Sci(国外医学脑血管疾病分册)[J],1996,4:326 |
| [4] | Tan V,Doyle C J,Bsc M.Am J Clin Pathol[J],1995,104(4):455 |
| [5] | Magnani B,Fenton T,Lapp C.Am J Clin Pathol[J],1997,107(5):527 |
| [6] | HE Ying-Wu(何英武),WU Rui-Ling(吴瑞玲).Modern J Integrated Trad Chin West Med(现代中西医结合杂志)[J],2004,13(18):2 511 |
| [7] | SHI Ji-Ren(施济人).Handbook of Clinical Laboratory(临床检验手册)[M].Ji′nan(济南):Shandong Science and Technology Press(山东科学技术出版社),1988:701 |
| [8] | HU Wang-Ping(胡望平),XIAO Wen-Hai(肖文海).Chinese J Clin Lab Sci(临床检验杂志)[J],1991,9(4):188 |
| [9] | TAO Xiang-Guang(陶相光).Chinese J Clin Lab Sci(临床检验杂志)[J],1993,11(4):187 |
| [10] | YE Ying-Wu(叶应妩).Operation Rule of National Clinical Laboratory(全国临床检验操作规程)[M].Nanjing(南京):Dongnan University Press(东南大学出版社),1991:146 |
| [11] | National Committee for Clinical Laboratory Standards.H30-Aprocedure for the Determination of Fibrinogen in Plasma;Approved Guideline.Pennsylvania:NCCLS,1999:99 |
| [12] | Exner T,Burridge J,Power P.Am Soc Clin Pathol[J],1979,71:521 |
| [13] | Koepke G A.Scand J Haematol Suppl[J],1980,37:130 |
| [14] | Hoegeede N E,Voskuilen M,Brilt E.Thromb Haemsot[J],1988,60:415 |
| [15] | Pasternack P F,Collings P J,Gianncttd A,Giannetto G E J.J Am Chem Soc[J],1993,115:5 393 |
| [16] | Huang C Z,Li Y F,Liu X D.Anal Chim Acta[J],1998,375:89 |
| [17] | Huang C Z,Li Y F,Shi Y D,Shu R L.Bull Chem Soc Jpn[J],1999,72:1 051 |
| [18] | Liu S P,Luo H Q,Li N B,Liu Z F.Anal Chem[J],2001,73:3 907 |
| [19] | Luo H Q,Liu S P,Liu Z F,Liu Q,Li N B.Anal Chim Acta[J],2001,449(1-2):261 |
| [20] | Ma C Q,Li K A,Tong S Y.Bull Soc Jpn[J],1997,70:129 |
| [21] | Guo C X,Shen H X.Anal Chim Acta[J],2000,408(1-2):177 |
| [22] | Jiang Z L,Liu S P,Liu Q Y.Talanta[J],2002,58:635 |
| [23] | JIANG Zhi-Liang(蒋治良),PAN Hong-Cheng(潘宏程),YUAN Wei-En(袁伟恩).Environ Chem(环境化学)[J],2004,23(5):573 |
| [24] | DENG Jun-Yao(邓俊耀),SUN Shuang-Jiao(孙双娇),JIANG Zhi-Liang(蒋治良),LIANG Ai-Hui(梁爱惠).Spectrosc Spect Anal(光谱学与光谱分析)[J],2006,26(8):1 487 |
| [25] | Liang A H,Jiang Z L,Zhang B M,Liu Q Y,Lan J,Lu X.Anal Chim Acta[J],2005,530(1):131 |
| [26] | PENG Li-Ming(彭黎明),ZENG Zhe-Li(曾着黎).Lab Med(检验医学)[J],2004,19(2):163 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%