以具柔性亲水手臂的壳聚糖-精氨酸树脂为载体,用戊二醛交联胰凝乳蛋白酶,获得壳聚糖-精氨酸树脂固定化胰凝乳蛋白酶. 最佳固定化条件为:m(酶)∶ m(载体)=20∶ 1 000、戊二醛体积分数为1.0%、pH=5.20、30 ℃交联60 min. 固定化酶活力达850 U/g,Km为1.83 mmol/L,比游离酶增大33.6%,比交联壳聚糖固定化酶低24.0%. 壳聚糖-精氨酸树脂固定化胰凝乳蛋白酶水解时间进程曲线与游离酶基本一致,均在反应30 min达到最大速率,最适温度为70 ℃,比游离酶升高10 ℃;在75 ℃时的半衰期可达6.0 h,比游离酶提高约4.3倍;最适pH值为5.92,比游离酶向酸性偏移2pH单位. 4 ℃贮存半衰期为49 d.
参考文献
| [1] | Harish Prashanth K V,Tharanathan R N.Trends Food Sci Technol[J],2007,18:117 |
| [2] | LIN Si-Chong(林思聪).Chinese Polym Bull(高分子通报)[J],1998,(1):1 |
| [3] | YUAN Chun-Tao(袁春桃),JIANG Xian-Ming(蒋先明).Chinese J Appl Chem(应用化学)[J],2002,19(9):862 |
| [4] | Anny Manrich,Célia M A,Galao,Charles D F Jesus,Roberto C Giordano,Raquel L C Giordano.Int J Bio Macromol[J],2008,43:54 |
| [5] | Jayakumari V G,Rajasekharan Pillai V N.J Appl Polym Sci[J],1991,42:583 |
| [6] | Hejazi R,Amiji M.Int J Pharm[J],2004,272:99 |
| [7] | Gabrovskaa K,Nedelchevaa T,Godjevargovaa T,Stoilovab O,Rashkov I.J Mol Catal B:Enzymatic[J],2008,55:169 |
| [8] | Xiao Y,Zhou X H.Reactive Func Polym[J],2008,68:1 281 |
| [9] | Frydlov(a) F,Kucerov(a) Z,TichK(a) M.J Chromatog B[J],2008,863:135 |
| [10] | Al-Shannag M,Al-Qodah Z,Herrero J,Humphrey J A C,Giralt F.Biochem Eng J[J],2008,39:554 |
| [11] | Reku A,Kruczkiewicz P,Jastrzembska B,Liesiene J,Peczy'nska-Czoch W,Bryjak J.Int J Bio Macromol[J],2008,42:208 |
| [12] | Chaijitrsakoola T,Tonanona N,Tanthapanichakoona W,Tamonb H,Prichanonta S.J Mol Catal B:Enzymatic[J],2008,55:137 |
| [13] | GAO Bo(高波),ZHU Guang-Shan(朱广山),FU Xue-Qi(付学奇),XIN Ming-Hong(辛明红),CHEN Jing(陈静),WANG Chun-Lei(王春雷),QIU Shi-Lun(裘式纶).Chem J Chinese Univ(高等学校化学学报)[J],2005,26(10):1 852 |
| [14] | Gabrovska K,Marinov I,Godjevargova T,Portaccio M,Lepore M,Grano V,Diano N.Int J Macromol[J],2008,43:339 |
| [15] | Godjevargova T,Gabrovska K.Enzyme Microbial Technol[J],2006,38:338 |
| [16] | NING Zheng-Xiang(宁正祥).Handbook of Food Component Analysis(食品成分分析手册)[M].Beijing(北京):Chinese Light Industry Press(中国轻工业出版社),1998:748,348 |
| [17] | Prakit Sukyai,Tonci Rezi,Cindy Lorenz,Kitti Mueangtoom,Werner Lorenz,Dietmar Haltrich,Roland Ludwig.J Biotechnol[J],2008,135:281 |
| [18] | YUAN Qin-Sheng(袁勤生),ZHAO Jian(赵健).Enzymes and Enzyme Engineering(酶与酶工程)[M].Shanghai(上海):East China University of Science and Technology Press(华东理工大学出版社),2005:172 |
| [19] | QI Yi-Zheng(戚以政),XIA Jie(夏杰).Biological Reaction Engineering(生物反应工程)[M].Beijing(北京):Chemical Industry Press(化学工业出版社),2004:25 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%