采用磷酸二异辛酯(P204)/正辛醇反胶束液从面包酵母粗提液中萃取还原型谷胱甘肽(GSH),研究了各种因素对萃取率的影响以及水相pH值和P204浓度与GSH萃取率关系的数学模型. 正交实验确定:GSH的最佳萃取条件是水相pH=2.0,K+浓度0 1 mol/L,萃取时间15 min,P204浓度0.16 mol/L. GSH单次的萃取率为33 1%,2次萃取率达49 7%.
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