经过4步反应合成了嘌呤核苷酸衍生物6-氯-2-氨基嘌呤核苷三磷酸钠盐,使用~1H NMR、~(13)C NMR、~(31)P NMR、IR和元素分析测试技术表征了其化学结构. 在不同pH值的溶液中研究了它的紫外光谱性质,并通过pH光度微量滴定法测定了一个质子化物种的pK_a=1.44. 最后,在马铃薯三磷酸腺苷双磷酸酶的催化下研究了该化合物的水解特性,该核苷酸衍生物与ATP竞争马铃薯三磷酸腺苷双磷酸酶的活性位点的机理被证明为混合型抑制机理.
A purine nucleotide derivative, 6-chloro-2-aminopurine nucleoside triphosphate, was synthesized in four steps and characterized with ~1H NMR, ~(31)P NMR, ~(13)C NMR, EA and IR. Its ultraviolet properties at various pH values were studied. The pK_a value of the compound was determined by the curves of UV abso-rption dependency on pH, which was 1.44. Hydrolysis of the nucleotide derivative under the catalysis of potato apyrase was studied. The competion of the ATP analogue with ATP for potato apyrase′ active site was proved to be mixed prohibition mechanism.
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