通过合成油酸修饰的Fe_3O_4纳米粒子和羧甲基壳聚糖直接包埋油酸修饰的Fe_3O_4纳米粒子的两步合成法制备了羧甲基壳聚糖磁性纳米粒子. 采用透射电子显微镜、傅里叶变换红外光谱、振动样品磁强计和同步热分析测试技术对制备的羧甲基壳聚糖磁性纳米粒子进行了表征. 所得磁性纳米粒子呈规则球形,粒径约为10 nm;表面含羧基,且具有很好的顺磁性和稳定性. 考察了羧甲基壳聚糖磁性纳米粒子对阿霉素的载药量和对阿霉素在磷酸盐缓冲溶液中的缓释性能. 结果表明,磁性纳米粒子对阿霉素展示了较高的载药量(91.8 mg/g),结合了阿霉素的磁性复合物对阿霉素的缓释作用明显,说明制备的羧甲基壳聚糖磁性纳米粒子有望作为治疗肿瘤的纳米磁靶向药物输送载体.
Carboxymethyl chitosan magnetic nanoparticles(CMCS-OA-Fe_3O_4 NPs) were prepared by a two-step method, which involved, synthesizing nanosized Fe_3O_4 in the presence of oleic acid(OA-Fe_3O_4 NPs) and directly coating the OA-Fe_3O_4 NPs with carboxymethyl chitosan in aqueous solution. The prepared CMCS-OA-Fe_3O_4 NPs were characterized with transmission electron microscopy(TEM), vibrating sample magnetometer(VSM), Fourier transform infrared spectrometry(FT-IR) and simultaneous thermal analyzer(STA), respectively. The CMCS-OA-Fe_3O_4 NPs were found to be regularly spherical with carboxyl on their surface, with a diameter of roughly 10 nm. The CMCS-OA-Fe_3O_4 NPs exhibited good paramagnetism and stability. The drug loading and the sustained-release property of the CMCS-OA-Fe_3O_4 NPs for adriamycin(ADM) in phosphate buffer solution(PBS, pH=7.4) were studied. High drug loading(91.8 mg/g) of CMCS-OA-Fe_3O_4 NPs and obvious sustained-release effect in vitro for ADM were reached. The results indicate that CMCS-OA-Fe_3O_4 NPs may be used as a magnetic-targeted nano drug carrier for the treatment of tumour.
参考文献
| [1] | Chandna P,Saad M,Wang Y,Ber E,Khandare J,Vetcher A A,Soldatenkov V A,Minko T.Mol Pharm[J],2007,4(5):668 |
| [2] | HE Qiang-Fang(何强芳),LI Guo-Ming(李国明),ZHU Jie-Min(朱洁民).Chinese J Appl Chem(应用化学)[J],2006,23(2):207 |
| [3] | Son S J,Reichel J,He B,Schuchman M.J Am Chem Soc[J],2005,127:7 316 |
| [4] | Kim J S,Rieter W J,Taylor K M L.J Am Chem Soc[J],2007,129:8 962 |
| [5] | Liang Y Y,Zhang L M.Biomacromolecules[J],2007,8:1 480 |
| [6] | HE Qiang-Fang(何强芳),LI Guo-Ming(李国明),WU Hai-Zhen(巫海珍).Chinese J Appl Chem(应用化学)[J],2004,21(2):192 |
| [7] | CHEN Yan(陈艳),SHEN He-Bo(沈鹤柏),XU Rui-Yun(徐瑞云).Chinese J Appl Chem(应用化学)[J],2008,25(5):564 |
| [8] | Wang Y S,Liu L R,Jiang Q,Zhang Q Q.Eur Polym J[J],2007,43:43 |
| [9] | XU Li-Ping(徐利平),CHEN Xiao-Ping(陈小萍).Chinese J Disinfect(中国消毒学杂志)[J],2005,22(1):96 |
| [10] | XIA Jin-Lan(夏金兰),LIAO Peng-Fei(廖鹏飞),NIE Zhen-Yuan(聂珍媛).J Cent South Univ Sci Technol(中南大学学报(自然科学版))[J],2006,37(6):1 075 |
| [11] | LIN Ben-Lan(林本兰),SHEN Xiao-Dong(沈晓冬),CUI Sheng(崔升).Inorg Chem Ind(无机盐工业)[J],2006,28(3):19 |
| [12] | YIN Xiang-Bao(殷香保),LI Hong-Hao(黎洪浩),CHEN Ru-Fu(陈汝福),YE Hua(叶华),REN Shan(任山),WANG Jie(王捷),QU Qing-Jia(区庆嘉).Chinese J Experim Surgery(中华实验外科杂志)[J],2006,23(2):227 |
| [13] | LIU Yu-Ling(刘瑜玲),CHEN Gai-Qing(陈改清),JING Wen-Tao(荆文涛),ZHANG Qiang(张强),HE Lei(何蕾),XU Liang(徐亮),ZHANG Shi-Yuan(张士元).Chinese J Practical Ophthalmology(中国实用眼科杂志)[J],2001,19(1):34 |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%