材料期刊网

H_2O_2在pH 9.8的NH_2·H_2O-NH_4Cl介质中产生的羟自由基可以迅速氧化结晶紫使其褪色,而血红蛋白模拟酶对褪色反应具有催化作用,据此建立了一种以结晶紫为指示剂的过氧化氢-结晶紫-血红蛋白酶催化反应体系测定痕量H_2O_2的新方法.研究了pH值,结晶紫,血红蛋白用量等因素对体系的影响.对雨水中Zn~(2+),Cu~(2+),Mg~(2+),Ca~(2+),Fe~(3+),Al~(2+)等常见离子进行了干扰试验.结果表明,除Fe~(3+)的允许量较小外,其他离子的允许量都较大;但是Fe~(3+)的干扰可以通过加入过量的柠檬酸消除.在最佳实验条件下,测定H~(2+)O~(2+)的线性范围为1.0×10~(-7)～3.0×10~(-5)mol/L,检出限为1.2×10~(-8)mol/L.该方法可用于测定雨水及消毒水中H_20_2的含量,结果与荧光法测定结果一致.

In NH_3-NH_4Cl at pH 9.8, the hydroxyl radical generated from H_2O_2 could rapidly oxidize crystal violet, while it was found that hemoglobin mimic enzyme had catalytic action on this fading reaction. Accordingly, a novel method for determination of trace hydrogen peroxide by hydrogen peroxide-crystal violet-hemoglobin enzyme catalyzed reaction system was established with crystal violet as indicator. The effects of pH, crystal violet and hemoglobin dosage on the determination were investigated. The interference of common ions in rain water including Zn~(2+) , Cu~(2+) , Mg~(2+) , Ca~(2+) , Fe~(3+) and Al~(3+) was tested. The results indicated that the tolerant amount of these ions were high except for Fe~(3+) which, however, could be eliminated by adding excessive citric acid. Under the optimal conditions, the linear range of H_2 O_2 was 1.0× 10~(-7)-3.0× 10~(-5) mol/L with the detection limit of 1.2× 10~(-8) mol/L. This method was suitable for the determination of hydrogen peroxide in rain water and disin-lector. The determination results were consistent with those obtained by fluorescent method.