为了研究新型膜材聚鸟氨酸对微囊化细胞功能的影响,制备了包埋有BRL细胞的聚鸟氨酸-海藻酸盐微胶囊并考察了微囊化肝细胞的生物学活性以及细胞功能.结果表明,载BRL细胞的PLO微胶囊形态完整,表面光滑,细胞在PLO微胶囊内的三维立体环境中能够维持活性,且具有维持分泌尿素和蛋白质的功能,从而为该新型膜材在细胞微囊化的应用方面提供了实验依据.
参考文献
| [1] | Lim F;Sun A M .Microencapsulated islets as bioartificial endocrine pancreas[J].SCIENCE,1980,210:908-910. |
| [2] | Yu W;Song H;Zheng G et al.Study on membrane characteristics of alginate-chitosan microcapsule with cell growth[J].Journal of Membrane Science,2011,377:214-220. |
| [3] | Kerby, A.;Jones, E.S.;Jones, P.M.;King, A.J. .Co-transplantation of islets with mesenchymal stem cells in microcapsules demonstrates graft outcome can be improved in an isolated-graft model of islet transplantation in mice[J].Cytotherapy,2013(2):192-200. |
| [4] | Pan X P;Li L J .Advances in cell sources of hepatocytes for bioartificial liver[J].Hepatobiliary & Pancreatic Dis eases International,2012,11:594-605. |
| [5] | S. Mert Selimoglu;Duygu Ayyildiz-Tamis;Ismet Deliloglu Gurhan .Purification of alginate and feasible production of monoclonal antibodies by thealginate-immobilized hybridoma cells[J].Journal of Bioscience and Bioengineering,2012(2):233-238. |
| [6] | Yen-Heng Lin;Ya-Wen Yang;Yi-Dao Chen .The application of an optically switched dielectrophoretic (ODEP) force for the manipulation and assembly of cell-encapsulating alginate microbeads in a microfluidic perfusion cell culture system for bottom-up tissue engineering[J].Lab on a chip,2012(6):1164-1173. |
| [7] | Wang S B;Liu Y G;Weng L J et al.Effect of molecular weights of poly-l-arginine on membrane strength and permeating property of poly-l-arginine group microcapsule[J].Macromolecular Bioscience,2003,3:347-350. |
| [8] | 刘源岗,王士斌,王莹.海藻酸钙-聚精氨酸聚电解质微胶囊强度性能研究[J].功能材料,2009(01):119-122. |
| [9] | Darrabie MD;Kendall WF;Opara EC .Characteristics of poly-L-ornithine-coated alginate microcapsules[J].Biomaterials,2005(34):6846-6852. |
| [10] | Tam SK;Bilodeau S;Dusseault J;Langlois G;Halle JP;Yahia LH .Biocompatibility and physicochemical characteristics of alginate-polycation microcapsules.[J].Acta biomaterialia,2011(4):1683-1692. |
| [11] | Ponce S;Orive G;Hernandez R;Gascon AR;Pedraz JL;de Haan BJ;Faas MM;Mathieu HJ;de Vos P .Chemistry and the biological response against immunoisolating alginate-polycation capsules of different composition.[J].Biomaterials,2006(28):4831-4839. |
| [12] | Gorka Orive;Rosa Maria Hernandez;Alicia R.Gascon;Manoli lgartua;Jose Luis Pedraz .Encapsulated cell technology from research to market[J].Trends in Biotechnology,2002(9):382-387. |
| [13] | Cukierman E;Pankov R;Yamada KM .Cell interactions with three-dimensional matrices [Review][J].Current Opinion in Cell Biology,2002(5):633-639. |
| [14] | Chia S M;Leong K W;Li J et al.Hepatocyte encapsulation for enhance cellular functions[J].Tissue Engineering,2000,6:481-495. |
| [15] | 陈耀凯,王宇明.生物人工肝的支架材料研究进展[J].生物医学工程学杂志,2003(01):153-156. |
| [16] | Hasan U;Paul D V;Partrick A T .Technology of mammalian cell encapsulation[J].Advanced Drug Delivery Reviews,2000,42:29-64. |
| [17] | Li JL.;Pan JL.;Zhang LG.;Yu YT. .Culture of hepatocytes on fructose-modified chitosan scaffolds[J].Biomaterials,2003(13):2317-2322. |
| [18] | 雄鹰 .海藻酸钠-壳聚糖-海藻酸钠微胶囊在细胞移植治疗中应用的研究[D].中国科学院大连化学物理研究所,2003. |
| [19] | Haque T;Chen H;Ouyang W;Martoni C;Lawuyi B;Urbanska AM;Prakash S .In vitro study of alginate-chitosan microcapsules: an alternative to liver cell transplants for the treatment of liver failure[J].Biotechnology Letters,2005(5):317-322. |
| [20] | 朱建航;张 宝;严喜鸾 等.细胞生理条件下壳聚糖微胶囊包埋肝细胞[J].生物医学工程杂志,2006,23:1070-1074. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%