采用共沉淀法制备乳酸插层MgAl 水滑石,在水中剥离后用于固载辣根过氧化物酶,制得水滑石固载酶,测试水滑石固载酶的稳定性和降解苯酚的性能。利用XRD、IR、UV-Vis等表征技术对制备的水滑石固载酶进行了表征,并对固载过程进行了分析。实验结果表明,加入磷酸缓冲溶液有利于剥离水滑石的固载,固载时剥离水滑石层板重构成磷酸根插层水滑石,而 H RP吸附在水滑石上。H RP 在水滑石上的固载过程符合拟一级动力学方程。由于水滑石具有无限延长的层板,为 H RP 的吸附提供了充足的表面积,在实验条件下,Langmuir 和Freundlich模型均较好地拟合了实验数据。H RP在水滑石上固载后,提高了其稳定性,新制备的固载酶处理苯酚的去除率在80%以上,循环使用3次后降解率依然可达50%左右。
Lactate intercalated layered double hydroxide (Lact-LDH )was prepared by co-precipitation,then it was delaminated in water.The delaminated LDH was used to immobilize horseradish peroxidase (HRP),and the stability and performance for the degradation on phenol by the resulted LDH immobilized HRP was investi-gated.It was shown that adding phosphate buffer solution was helpful to the immobilization and the delamina-ted LDHs were reconstructed to phosphate intercalated LDHs,on which surfaces the HRPs were adsorbed. The immobilization process could be described by the pseudo-first-order kinetics model.Under the experimental conditions,both Langmuir and Freundlich isotherms fitted the experimental data,owing to the infinite surfaces of LDH.The stability of HRP was improved after being immobilized on LDH.The phenol removal could reach above 80% on the as-prepared immobilized HRP and still kept 50% after 3 cycles usage.
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