血管支架的制备成为治疗心血管疾病的必需条件。应用静电纺丝技术制备了(pNSR16/PCL/CS)/(pNSR16/PCL/Gt)双层血管支架,并研究其在磷酸盐缓冲液(pH 值=7.4)和多酶降解液中浸泡不同时间的体外降解情况。于2、4、8、12周分别取材,测试失重率、吸水率、降解液的 pH 值、力学性能和分子量的变化,并进行扫描电镜观察。结果表明,(pNSR16/PCL/CS)/(pNSR16/PCL/Gt)双层血管支架的初始抗弯强度和初始分子量大于(PCL/CS )/(PCL/Gt)支架,并且前者的降解速度、分子量和失重率减少速度快于后者,pNSR16的添加促进了血管支架的降解。在降解过程中,浸泡液 pH 值呈弱酸性和中性,前期迅速降低并在后期呈现稳定的趋势。支架在酶解液中的降解速度快于在水解液中。
The preparation of vascular scaffold becomes a necessary condition for cure for cardiovascular disease. In this paper electrospinning technique was used to prepare the (pNSR1 6/PCL/CS)/(pNSR1 6/PCL/Gt)bilayer vascular scaffold,and its degradation in vitro was studied after being soaked in phosphate buffer solution (pH=7.14)and multiple enzyme solution for different time.The changes of weight loss rate,water absorption rate, pH value of degradation solution,mechanical property and molecular weight were tested after sampling at 2nd, 4th,8ht,12th week respectively,and at the same time the morphology was observed using scanning electron microscope.The results showed that the initial bending strength and molecular weight of (pNSR1 6/PCL/CS)/(pNSR1 6/PCL/Gt)bilayer vascular scaffold were greater than those of (PCL/CS)/(PCL/Gt),the degradation velocity and decreasement velocity of molecular weight and weight loss rate of the former were faster than the latter,and the addition of pNSR1 6 promoted the degradation of vascular scaffold.In the process of degradation, pH value of degradation solution was weakly acidic and neutral,the scaffold decreased rapidly in the prophase and showed stable trend in the late period.The degradation speed of scaffold in the enzyme solution was faster than it in the hydrolysis soultion.
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