制备一种新的硬度可变的生物基底材料。通过红外光谱对比分析、吸水称重法、旋转流变仪法、MTT法等实验对壳聚糖/聚丙烯酰胺水凝胶材料的结构、溶胀性、弹性模量、细胞毒性等性能进行测定。将HepG2细胞接种到壳聚糖/聚丙烯酰胺水凝胶基底材料上,24h后对材料上生长的细胞进行形态学观察比较细胞的生长情况。结果发现不同配方制成的壳聚糖/聚丙烯酰胺水凝胶的硬度具有明显梯度差异,具有良好的生物相容性。这种新的生物基底材料可以为细胞力学基础研究提供帮助。
A new type of hardness variable biological substrate material was prepared.Experiments of infrared spectroscopy,water absorption of weighing,the rotational rheometer,and MTT were conducted to measure the structure,swell ability,elastic modulus and cytotoxicity of polyacrylamide/chitosan hydrogel HepG2 cells were inoculated into the mixed hydrogel base material of polyacrylamide/chitosan,and the morphology of the cells grown on the materials was observed after 24h.The hardness of the different formulation of polyacrylam-ide/chitosan hydrogel had obvious gradient differences and good biocompatibility.This new type of the biologi-cal substrate material could contribute to the research of cell mechanics.
参考文献
| [1] | Rajagopalan, P;Marganski, WA;Brown, XQ;Wong, JY .Direct comparison of the spread area, contractility, and migration of balb/c 3T3 fibroblasts adhered to fibronectin- and RGD-modified substrata[J].Biophysical Journal,2004(4):2818-2827. |
| [2] | Radmacher M;Fritz;Kacher C M et al.Measuring the viscoelastic properties of human platelets with the atomic force microscope[J].BIOPHYSICAL JOURNAL,1996,70(01):556-567. |
| [3] | Lorenz, B;Mey, I;Steltenkamp, S;Fine, T;Rommel, C;Muller, MM;Maiwald, A;Wegener, J;Steinem, C;Janshoff, A .Elasticity Mapping of Pore-Suspending Native Cell Membranes[J].Small,2009(7):832-838. |
| [4] | Docheva D;Padula D;Schieker M;Clausen Schaumann H .Effect of collagen I and fibronectin on the adhesion, elasticity and cytoskeletal organization of prostate cancer cells.[J].Biochemical and Biophysical Research Communications,2010(2):361-366. |
| [5] | Engler AJ;Sen S;Sweeney HL;Discher DE .Matrix elasticity directs stem cell lineage specification[J].Cell,2006(4):677-689. |
| [6] | Stroka KM;Aranda Espinoza H .Neutrophils display biphasic relationship between migration and substrate stiffness.[J].Cell motility and the cytoskeleton,2009(6):328-341. |
| [7] | Engler AJ;Griffin MA;Sen S;Bonnetnann CG;Sweeney HL;Discher DE .Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments[J].The Journal of Cell Biology,2004(6):877-887. |
| [8] | Gaudet C;Marganski W A;Kim S et al.Influence of type I collagen surface density on fibroblast spreading,motility,and contractility[J].BIOPHYSICAL JOURNAL,2003,85:3329-3335. |
| [9] | Lo,CM;Wang,HB;Dembo,M;Wang,YL .Cell movement is guided by the rigidity of the substrate.[J].Biophysical Journal,2000(1):144-152. |
| [10] | Pelham R J Jr;Wang Y I .Cell locomotion and focal ad-hesions are regulate by substrate flexibility[J].Proceedings of the National Academy of Sciences(USA),1997,94:13661-13665. |
| [11] | Winer JP;Janmey PA;McCormick ME;Funaki M .Bone marrow-derived human mesenchymal stem cells become quiescent on soft substrates but remain responsive to chemical or mechanical stimuli.[J].Tissue engineering, Part A,2009(1):147-154. |
| [12] | Yeung T;Georges PC;Flanagan LA;Marg B;Ortiz M;Funaki M;Zahir N;Ming W;Weaver V;Janmey PA .Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion.[J].Cell motility and the cytoskeleton,2005(1):24-34. |
| [13] | Solon J;Levental I;Sengupta K;Georges PC;Janmey PA .Fibroblast adaptation and stiffness matching to soft elastic substrates[J].Biophysical Journal,2007(12):4453-4461. |
| [14] | 章汝平,丁马太.聚乙烯醇/壳聚糖共混膜的制备及表征[J].功能材料,2007(12):2004-2007. |
| [15] | 王家鸣,任力,龙玉宇,刘卅,王迎军,季培红,林明楷,席蕾.壳聚糖-胶原复合膜渗透性能的研究[J].功能材料,2012(20):2747-2750. |
| [16] | 周旋,孔明,刘卫芳,冯超,程晓杰,陈西广.乙酰化壳聚糖微球作为潜在动脉栓塞剂的研究[J].功能材料,2012(18):2478-2482. |
| [17] | 宋晓艳,邢金峰,邓联东,董岸杰.双氯灭痛壳聚糖水凝胶微球的制备及其性能研究[J].功能材料,2010(01):105-107. |
| [18] | 张利,李玉宝,杨爱萍,左奕,吕国玉,魏杰.骨组织工程用纳米羟基磷灰石/ 壳聚糖多孔支架材料的制备及性能表征[J].功能材料,2005(02):314-317. |
| [19] | 叶芬,尹玉姬,孙光洁,宋雪峰,姚康德.壳聚糖支架在组织工程中的应用[J].功能材料,2002(05):459-461. |
| [20] | 熊轶,张巨峰,刘文丽.神经干细胞三维空间壳聚糖生物材料中培养[J].现代生物医学进展,2010(08):1414-1415,1419. |
| [21] | 谭太贵,马景鑑,张文治,苏心,王立凯,冯喜增.神经干细胞在PLGA、壳聚糖和明胶膜表面的生长规律[J].中国生物医学工程学报,2006(03):377-381. |
| [22] | Wang Y L;Pelham R J .Preparation of a flexible,por-ous polyacrylamide substrate for mechanical studies of cultured cells[J].Methods in Enzymology,1998,298:489-496. |
| [23] | 李玉宝.生物医学材料[M].北京:化学工业出版社,2003:279. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%